摘要
市售人工肌腱-韧带移植物大多采用聚对苯二甲酸乙二醇酯(PET)纤维作为基体材料,但因PET缺乏骨诱导活性易导致腱-骨愈合差的问题。本文采用促骨活性功能粒子——纳米羟基磷灰石(nano-HAp)作为功能组分,借助纳米杂化熔融纺丝技术制备系列nano-HAp负载量的PET/HAp杂化纤维,然后采用机织技术制备了补片形式的PET/HAp杂化纤维基移植物,从形貌、结构、力学性能、细胞相容性等方面系统研究了nano-HAp的引入对PET/HAp杂化材料的影响。结果表明:借助双螺杆共混造粒和复配熔融纺丝工艺,成功制得系列有效负载nano-HAp(0%,0.5%和1.5%(质量分数))的PET/HAp杂化纤维,但因其与PET的相容性较差,nano-HAp添加量为质量分数1.5%时,PET/HAp纤维的力学性能和结晶度明显降低,但制备的杂化纤维断裂强度(≥3 cN/dtex)均满足机织要求;利用PET/HAp杂化纤维编织的补片,其断裂强度((34.6±0.9)MPa)满足替代人体天然肌腱组织(肩袖)的力学强度;体外与成骨细胞(MC3T3-E1)的共培养实验表明,PET/HAp杂化纤维基补片(Patch)具有良好的细胞相容性;体外矿化实验表明,nano-HAp的加入可有效提高纤维基补片上矿化物的沉积,其中nano-HAp负载量为质量分数1.5%杂化纤维所织的补片(Patch-1.5)具有最佳的矿化沉积性能,因此,PET/HAp杂化纤维有望用于促进肌腱韧带修复中的腱-骨愈合。
The majority of commercially available artificial tendon-ligament grafts are fabricated using polyethylene terephthalate(PET)fibers as the matrix material.However,the lack of bone inductive activity in PET can lead to poor tendon-to-bone healing.This study introduces bone-promoting functional particles—nano-hydroxyapatite(nano-HAp)—as a functional component.A series of PET/HAp hybrid fibers with varying nano-HAp loading levels were prepared using nano-hybrid melt spinning technology.Subsequently,patch-shaped PET/HAp hybrid fiber-based grafts were fabricated using weaving technology.The introduction of nano-HAp into PET/HAp hybrid materials was systematically investigated regarding morphology,structure,mechanical properties,and cell compatibility.Experimental results indicate that,through twin-screw compounding granulation and co-extrusion melt spinning,a series of PET/HAp hybrid fibers with effective nano-HAp loading(0%,0.5%and 1.5%)were produced.However,due to poor compatibility with PET,the mechanical properties and crystallinity of PET/HAp fibers noticeably decreased when the nano-HAp loading level reached 1.5%.Nonetheless,the fracture strength(≥3 cN/dtex)of the prepared hybrid fibers met weaving requirements.Weaving PET/HAp hybrid fibers into patches resulted in the fracture strength((34.6±0.9)MPa)that met the mechanical strength of replacing human natural tendon tissue(rotator cuff).In vitro co-culture experiments with osteoblasts(MC3T3-E1)demonstrated good cell compatibility of PET/HAp hybrid fiber-based patches(Patch).In vitro mineralization experiments revealed that the addition of nano-HAp effectively enhanced the deposition of minerals on the fiber-based patches.Among them,the patch woven from hybrid fibers with a nano-HAp loading of 1.5%(Patch-1.5)exhibited the best mineral deposition performance.Consequently,PET/HAp hybrid fibers hold promise for promoting tendon-to-bone healing in tendon-ligament repairs.
作者
胡慧
陆腱
费翔
Hu Hui;Lu Jian;Fei Xiang(College of Materials Science and Engineering,Donghua University,Shanghai 201600,China)
出处
《山东化工》
CAS
2024年第17期64-68,77,共6页
Shandong Chemical Industry
作者简介
胡慧,女,硕士,研究方向:生物医用材料;通信作者:陆腱,博士,博士后,研究方向:生物医用材料;通信作者:费翔,博士,教授,研究方向:生物医用材料。
