摘要
为探究枯草芽孢杆菌在盐胁迫下降解组胺的机制,以枯草芽孢杆菌JZXJ-7为试验菌株,在添加170 mmol/L和340 mmol/L NaCl的液态培养环境中培养48 h,通过测定组胺降解率和菌落生长情况,同时对降解菌株进行转录组高通量测序,筛选不同NaCl添加组的菌株在降胺过程中的差异基因表达和相关代谢通路分析。结果表明,添加170 mmol/L和340 mmol/L NaCl后组胺的降解率显著高于空白组(P<0.05),分别为62.93%和84.87%;菌落总数显著增加(P<0.05),分别为4.13×10^(5)CFU/mL和4.53×10^(5)CFU/mL。转录组分析发现,盐胁迫组的差异表达基因显著上调,主要上调功能基因为丝氨酸乙酰转移酶、主协同转运蛋白超家族(MFS)转运蛋白和I型分泌系统渗透酶/ATP酶,显著下调的功能基因有组氨酸激酶、II型3-脱氢酸脱水酶和莽草酸脱氢酶。GO功能富集分析主要涉及代谢过程、细胞转化、细胞解剖实体、结构分子活性、催化活性和结合能力等。KEGG代谢途径涉及丙酮酸代谢、磷酸戊糖途径、双组分系统和精氨酸生物合成等。研究结果说明,盐胁迫更有利于枯草芽孢杆菌促进基因表达,激活代谢通路,提高组胺降解率。本研究为后续菌株应用于食品发酵行业,进一步高效降解生物胺提供重要的理论基础和参考依据。
In order to explore the mechanism of histamine degradation of Bacillus subtilis under salt stress,B.subtilis JZXJ-7 was used as the experimental strain,and cultured in liquid culture environment with 170 mmol/L and 340 mmol/L NaCl for 48 h.Histamine degradation rate and colony growth were measured,and transcripome high-throughput sequencing was performed on the degraded strain.The differential gene expression and related metabolic pathways of strains in different NaCl addition groups were detected during reducing amine process.Results showed that the degradation rate of histamine after adding 170 mmol/L and 340 mmol/L NaCl was significantly higher than control group(P<0.05),which were 62.93%and 84.87%,respectively.The total number of colonies was significantly increased(P<0.05),which were 4.13×10^(5) CFU/mL and 4.53×10^(5) CFU/mL,respectively.Transcriptome analysis showed that differentially expressed genes were significantly up-regulated in the salt stress group,and the main up-regulated functional genes were serine acetyltransferase,major facilitator superfamily(MFS)transporter and type I secretory system penetrase.The significantly down-regulated functional genes were histidine kinase,type II 3-dehydroate dehydrase and shikimic dehydrogenase.GO functional enrichment analysis mainly involved metabolic process,cell transformation,cell anatomical entity,structural molecular activity,catalytic activity and binding ability.The metabolic pathway of KEGG involved pyruvate metabolism,pentose phosphate pathway,two-component system and arginine biosynthesis.Results revealed that salt stress was more conducive to B.subtilis to promote gene expression,activate metabolic pathways,and increase histamine degradation rate.This study provided an important theoretical basis and reference for the subsequent strains to be applied in food fermentation industry to further degrade biogenic amines efficiently.
作者
邓义佳
王润东
檀茜倩
李学鹏
张宇昊
张宾
励建荣
Deng Yijia;Wang Rundong;Tan Xiqian;Li Xuepeng;Zhang Yuhao;Zhang Bin;Li Jianrong(College of Food Science and Engineering,Bohai University,Jinzhou 121013,Liaoning;College of Food Science,Southwest University,Chongqing 400715;Chongqing Key Laboratory of Speciality Food Co-Built by Sichuan and Chongqing,Chongqing 400715;College of Food and Pharmacy,Zhejiang Ocean University,Zhoushan 316022,Zhejiang)
出处
《中国食品学报》
EI
CAS
CSCD
北大核心
2024年第8期435-447,共13页
Journal of Chinese Institute Of Food Science and Technology
基金
“十三五”国家重点研发计划蓝色粮仓科技创新重点专项(2019YFD0901702)
国家自然科学基金项目(32201929
32202134)
2022年重庆市博士后特别资助项目。
关键词
枯草芽孢杆菌
组胺
转录组
降解率
差异基因表达
Bacillus subtilis
histamine
transcriptome
degradation rate
differential gene expression
作者简介
第一作者:邓义佳,女,博士;通信作者:张宇昊,E-mail:zhy1203@163.com;通信作者:励建荣,E-mail:lijr6491@163.com。
