摘要
为比较分析我国传统药食同源植物,选取103种药食两用植物,根据外观将其分为果实、花叶、全草、种子、皮、根茎、真菌和其它类,测定其总酚和总黄酮含量,评价其体外抗氧化活性、α-葡萄糖苷酶和乙酰胆碱酯酶抑制能力,并采用高效液相色谱分析其化学成分。结果表明:各药食两用植物的总酚和总黄酮含量差异较大,其中总酚含量以花叶类的丁香(309.59μg GAE/mg)、果实类的青果(229.64μg GAE/mg)最高,总黄酮含量以全草类的蒲公英(662.31μg RE/mg)、小蓟(505.33μg RE/mg)最高。相对而言,花叶、果实与全草类药食两用植物具有良好的的体外抗氧化活性。此外,果实类的覆盆子和青果、皮类的肉桂、花叶类的丁香显示出较好的α-葡萄糖苷酶抑制能力,而根茎类的姜黄、皮类的肉桂、全草类的香薷对乙酰胆碱酯酶的抑制能力较强,说明这些药食两用植物具有较好地预防糖尿病与阿尔茨海默病的潜力。高效液相色谱分析表明:从花叶类、果实类与全草类药食两用植物中检出的化合物种类较多,其中儿茶素、绿原酸与表儿茶素等是主要化合物,与抗氧化活性呈显著正相关关系,它们可能是这些药食两用植物抗氧化的主要活性物质。本文首次比较分析103种传统药食两用植物的化学成分和功能活性,对这些植物在居民膳食指导和临床治疗中的应用具有一定的参考价值。
In order to conduct a comparative analysis of traditional medicine-food homology ingredients in China,103 medicinal and edible plants(MEPs)were selected and divided into eight categories based on their appearance.The study evaluated the following properties of MEPs:Total phenolic content(TPC),total flavonoid content(TFC),in vitro antioxidant activity andα-glucosidase and acetylcholinesterase inhibition.Furthermore,their chemical constituents were identified by high-performance liquid chromatography(HPLC).The results showed significant differences in TPC and TFC among MEPs,where Flos caryophylli in the mosaic category and Fructus canarii in the fruit category had the highest TPC with values of 309.59μg GAE/mg and 229.64μg GAE/mg.For TFC,Taraxacum mongolicum Hand.-Mazz.and Cirsium setosum in the whole grass category were the highest(662.31μg RE/mg and 505.33μg RE/mg).Meanwhile,mosaic,fruit,and whole grass category had good antioxidant activities.In addition,Rubus chingii and Fructus canarii in the fruit category,Cinnamomum cassia Presl in the derma category,and Flos caryophylli in the mosaic category demonstrated strongα-glucosidase inhibition activity.While turmeric in the rhizome category,Cinnamomum cassia Presl in the derma category,Herba moslae in the whole grass category exerted a remarkable inhibitory activity against acetylcholinesterase.HPLC analysis revealed that the mosaic,fruit,and whole grass categories had the most abundant compound species.In addition,catechin,chlorogenic acid,and epicatechin were the main compounds and had a significant positive correlation with antioxidant activity,It may be the main active substance of antioxidant in these medicinal and edible plants.This is the first comparative analysis on the chemical constituents and functional activities of 103 traditional MEPs,which can provide a valuable reference for the application of the clinical therapy and resident dietary guidance of these MEPs.
作者
刘晓海
茹月蓉
张雪春
周旭红
何霞红
王振兴
Liu Xiaohai;Ru Yuerong;Zhang Xuechun;Zhou Xuhong;He Xiahong;Wang Zhenxing(College of Biological Science and Food Engineering,Southwest Forestry University,Kunming 650224;Research and Experiment Center,Yunnan University of Chinese Traditional Medicine,Kunming 650500;Yunnan Provincial Key Laboratory for Conservation and Utilization of In-forest Resource,Key Laboratory for Forest Resources Conservation and Utilization in the Southwest Mountains of China,Ministry of Education,Kunming 650224)
出处
《中国食品学报》
EI
CAS
CSCD
北大核心
2024年第8期385-402,共18页
Journal of Chinese Institute Of Food Science and Technology
基金
云南省中央引导地方科技发展资金项目(202207 AB110015)
云南省“兴滇英才支持计划”创业人才项目(2020)
云南省“兴滇英才支持计划”青年人才项目(2022,2023)
国家中药材产业技术体系岗位项目(CARS-21-05B)
云南省郑文杰专家工作站项目(202205AF150018)。
作者简介
第一作者:刘晓海,男,硕士生;通信作者:何霞红,E-mail:hexiahong@hotmail.com;通信作者:王振兴,E-mail:wangzhenxingfood@163.com。
