摘要
板栗内腐病是板栗采后贮藏期主要病害,引起栗仁腐烂霉变,给生产带来巨大经济损失。为明确板栗内腐病的致病菌及其生物学特性,采用组织分离法获得菌株ZHZF21,通过形态学特征及ITS、TUB2、CAL多基因序列分析对该菌株进行鉴定,以菌丝块有伤接种法测定菌株ZHZF21的致病性,并对其生物学特性进行研究。结果表明,菌株ZHZF21的菌落呈墨绿色环状同心圆圈,具备有性和无性繁殖阶段。该菌株的序列与GenBank中编号为NDSTY31的果生炭疽菌(Colletotrichum fructicola)聚为一支。结合菌落形态特征及分子树将ZHZF21初步鉴定为果生炭疽菌。将菌株ZHZF21接种栗果后产生褐色病斑,与自然发病症状一致。该菌株在5~35℃均可生长,最适生长温度为25℃,致死温度为45~50℃,最适pH为6;在全黑暗条件下生长最快,阿拉伯树脂糖和酵母的利用率最高,乳糖的利用率最低,几乎不能利用尿素。
Chestnut internal rot is the main disease of chestnut during postharvest storage,which causes rot and mildew of chestnut kernel and brings huge economic losses to production.In order to clarify the pathogenic bacteria and its biological characteristics,the strain ZHZF21 was obtained by tissue isolation method.The strain was identified by morphological characteristics and ITS,TUB2 and CAL multi-gene sequence analysis.The pathogenicity of strain ZHZF21 was determined by mycelial inoculation method,and its biological characteristics were studied.The results showed that the colony of strain ZHZF21 was dark green ring concentric circles,with sexual and asexual reproduction stages.This strain was clustered with Colletotrichum fructicola numbered NDSTY31 in GenBank based on multi-gene sequences.Combined with colony morphological characteristics and molecular tree,strain ZHZF21 was preliminarily identified as Colletotrichum fructicola.The brown lesions after inoculation of strain ZHZF21 were consistent with the natural symptoms.The strain ZHZF21 could grow at 5~35℃,the optimum temperature was 25℃,the lethal temperature was 45~50℃,and the optimum pH was 6.Under the condition of full darkness,strain ZHZF21 grew fastest,the utilization rates of arabinose and yeast were the highest,the utilization rate of lactose was the lowest,and urea could hardly be used.
作者
母时风
温晓蕾
冯丽娜
赵德轩
高素红
高朋
齐慧霞
MU Shifeng;WEN Xiaolei;FENG Lina;ZHAO Dexuan;GAO Suhong;GAO Peng;QI Huixia(Chestnut Industry Technology Engineering Research Center of Ministry of Education,Hebei Qinhuangdao 066004,China;Hebei Key Laboratory of Crop Adversity Biology,College of Agronomy and Biotechnology,Hebei Normal University of Science and Technology,Hebei Changli 066600,China)
出处
《中国农业科技导报》
CAS
CSCD
北大核心
2024年第9期122-128,共7页
Journal of Agricultural Science and Technology
基金
河北省板栗产业协同创新中心项目(2022—2023)。
关键词
板栗
内腐病
病原菌鉴定
果生炭疽菌
生物学特性
chestnut
internal rot
pathogen identification
colletotrichum fructicola
biological characteristics
作者简介
共同第一作者:母时风,E-mail:2786848750@qq.com;共同第一作者:温晓蕾,E-mail:xiaoleiwen@sina.com;通信作者:齐慧霞,E-mail:qihuix@163.com。
