摘要
为了对倒刺狗尾草叶绿体基因组的基本特征和系统发育进行分析,本研究对倒刺狗尾草的叶绿体基因组进行了测序,并对测序结果进行组装、注释和特征分析,并以拟南芥为外类群,利用15种已知叶绿体基因组的禾本科植物构建系统发育树。结果表明,倒刺狗尾草叶绿体基因组总长度为138946 bp,总GC含量为38.5%,共注释得到131个基因,包括83个CDS基因、40个tRNA基因和8个rRNA基因。密码子偏向使用A/T结尾的密码子。倒刺狗尾草检测到60个简单重复序列(SSR),A-T重复较多。通过系统发育分析得知,倒刺狗尾草与蒺藜草亲缘关系最为密切。
In order to analyze the basic characteristics and phylogeny of the chloroplast genome of Setaria verticillata,in this study the chloroplast genome of Setaria verticillata was sequenced,with the sequencing results assembled,annotated and characteristics analysed.In addition,15 species of grasses with known chloroplast genomes were used to construct phylogenetic trees with Arabidopsis thaliana as exotaxa.The results showed that the total length of chloroplast genome was 138946 bp,the total GC content was 38.5%,and a total of 131 genes were annotated,including 83 CDS genes,40 tRNA genes and 8 rRNA genes.Codons tend to use codons ending in A/T.60 simple repeat sequences(SSRS)were detected in Setaria verticillata,and A-T repeats were more frequent.Phylogenetic analysis showed that Setaria verticillata was most closely related to Cenchrus echinatus.
作者
刁玉霖
李竑阳
张海金
张文飞
王凯玺
朱康宁
张蒙
孙鑫
李前
陈国秋
DIAO Yulin;LI Hongyang;ZHANG Haijin;ZHANG Wenfei;WANG Kaixi;ZHU Kangning;ZHANG Meng;SUN Xin;LI Qian;CHEN Guoqiu(Liaoning Institute of Dryland Agriculture and Forestry,Chaoyang Liaoning 122000,China;Yuxi Agricultural Technology Extension Station of Yunnan Province,Yuxi Yunnan 653100,China)
出处
《种子》
北大核心
2024年第8期45-51,59,共8页
Seed
基金
国家现代农业产业技术体系(CARS-06-14.5-B15)
辽宁省民生计划项目(2021JH2/10200029)
辽宁省建平县杂粮产业乡村振兴科技特派团(2023020322-JH5/104)
谷子产业提升技术示范推广(2024-XCZX-A2-72)
辽宁省农业科学院院长基金项目(2024QN1813)。
作者简介
刁玉霖(1992-),男(汉族),辽宁大连人,硕士,助理研究员,主要从事谷子育种与栽培工作(E-mail:1095596434@qq.com);通讯作者:陈国秋(1970-),女(汉族),辽宁朝阳人,硕士,研究员,主要从事谷子育种与栽培工作(E-mail:chen13019935617@126.com)。
