摘要
为了解不同品种肉鸽胸肌组织的代谢差异及其对肉品质和风味产生的影响,选取28日龄银王鸽和申王肉鸽1号乳鸽各16只,采用液相色谱-质谱联用(LC-MS)技术对其胸肌代谢物进行非靶向代谢检测,并运用代谢组学分析技术进行代谢模式差异分析、差异代谢物筛选和差异代谢物代谢通路富集分析。结果表明:在正、负离子模式下,银王鸽和申王肉鸽1号胸肌中分别检测出80种和47种代谢物,筛选出13种差异代谢物。与银王鸽相比,申王肉鸽1号中有8种代谢物丰度显著上调(P<0.05),5种显著下调(P<0.05)。KEGG分析表明:这13种差异代谢物在23条代谢通路上富集,主要为氨基酸代谢、不饱和脂肪酸代谢、核苷酸代谢、磷酸盐代谢等通路。研究结果可为进一步阐明不同鸽品种中胸肌风味物质的调控机制提供科学依据。
In order to understand the metabolic differences in the pectoral muscle tissue of different meat pigeons and their effects on meat quality and flavor,16 Silver King pigeons and 16 Shenwang No.1 meat pigeons aged 28 days were selected,non-targeted metabolites of pectoral muscle metabolites were detected by liquid chromatoc-mass spectrometry(LC-MS),and metabolomics analysis technique was used for differential analysis of metabolic patterns,screening of differential metabolites,and enrichment analysis of differential metabolite metabolic pathways.The results showed that under positive/negative ion modes,80 and 47 metabolites were detected in pectoral muscle of Silver King pigeons and Shenwang No.1 pigeons,and 13 differential metabolites were identified.Compared to Silver King pigeons,8 differential metabolites were up-regulate significantly(P<0.05)and 5 were down-regulate significantly(P<0.05)in Shenwang No.1 pigeons.KEGG analysis showed that these 13 differential metabolites were enriched on 23 metabolic pathways,mainly including amino acid metabolism,unsaturated fat metabolism,nucleotide metabolism,phosphate metabolism and other metabolic pathways.Scientific basis for further elucidating the regulatory mechanism of chest muscle flavor substances were provided in different pigeon varieties.
作者
沈晓晖
高瑞圆
侯浩宾
李欣
丁卫星
杨长锁
赵维民
姚俊峰
SHEN Xiaohui;GAO Ruiyuan;HOU Haobin;LI Xin;DING Weixing;YANG Changsuo;ZHAO Weimin;YAO Jumnfeng(Shanghai Academy of Agricultural Sciences,Shanghai 201106,China;Shandong Agricultural University,Taian 271000,China;National Poultry Engineering Technology Research Center,Shanghai 201106,China;Shanghai Jinhuang Industry Co.,Ltd.,Shanghai 201505,China)
出处
《上海农业学报》
2024年第4期67-73,共7页
Acta Agriculturae Shanghai
基金
上海市肉鸽产业技术体系[沪农科产字(2022)第12号]
上海市农业科学院卓越团队[沪农科卓(2022)021]。
作者简介
沈晓晖(1979-),男,博士,高级畜牧师,研究方向为家禽饲养管理。E-mail:shenxiaohui@saas.sh.cn;共同第一作者:高瑞圆,E-mail:1243774746@qq.com;通信作者:姚俊峰,E-mail:yaobison@163.com。
