摘要
【目的】通过生物信息学方法初步筛选与滇山茶花色相关的bHLH基因,为后续深入研究滇山茶花色提供支持。【方法】基于滇山茶全长转录组数据,通过生物信息学方法鉴定滇山茶bHLH基因家族成员,并对其理化性质、二级结构、系统发育关系和结构域等进行分析。利用二代转录组和代谢组数据挖掘与滇山茶花色相关的bHLH基因,通过实时定量聚合酶链式反应(real-time quantitative polymerase chain reaction,RT-qPCR)验证bHLH基因在滇山茶开花过程中和不同品种间的表达情况。【结果】在滇山茶全长转录组中鉴定到71个滇山茶CrbHLHs基因,蛋白分子量的范围在22994.58-102996.96 Da,等电位为4.98-9.51,均为亲水性蛋白。与拟南芥聚类分析发现,滇山茶bHLH基因家族成员可分布到14个亚族。多组学联合分析发现,CrbHLH8、CrbHLH61和CrbHLH63与矢车菊色素苷呈正相关,而CrbHLH59与其呈负相关;CrbHLH13和CrbHLH71与原花青素呈正相关,其中CrbHLH8和CrbHLH61为IIIf亚族成员。【结论】从滇山茶全长转录组中鉴定出71个CrbHLHs家族成员,其中6个CrbHLHs与滇山茶花色相关。
【Objective】The preliminary screening of bHLH genes related to C.reticulata flower color by bioinformatics method provides support for further study of C.reticulata flower color.【Method】Based on the full-length transcriptome data of C.reticulata,the members of the bHLH gene family were identified by bioinformatics method,and their physical and chemical properties,secondary structure,phylogenetic relationships and domains were analyzed.The second-generation transcriptome and metabolome data were used to mine the bHLH genes related to the flower color of C.reticulata.Real-time quantitative polymerase chain reaction(RT-qPCR)was used to verify the expression of bHLH gene during flowering of C.reticulata among different varieties.【Result】Total 71 CrbHLHs genes were identified in the full-length transcriptome of C.reticulata.Their molecular weight ranged from 22994.58 to 102996.96 Da,and their equipotential ranged from 4.98 to 9.51.All of them were hydrophilic proteins.The cluster analysis with Arabidopsis thaliana showed that the bHLH gene family members of C.reticulata could be distributed into 14 subfamilies.Multiple omics analysis showed that CrbHLH8,CrbHLH61 and CrbHLH63 were positively correlated with cyanidin,while CrbHLH59 was negatively correlated with cyanidin.CrbHLH13 and CrbHLH71 were positively correlated with proanthocyanidins,and CrbHLH8 and CrbHLH61 were members of the IIIf subfamily.【Conclusion】The 71 CrbHLHs family members were identified from the full-length transcriptome of C.reticulata,of which 6 CrbHLHs were related to C.reticulata flower color.
作者
周麟
黄顺满
苏文坤
姚响
屈燕
ZHOU Lin;HUANG Shun-man;SU Wen-kun;YAO Xiang;QU Yan(College of Landscape and Horticulture,Southwest Forestry University,Southwest Research Center for Engineering Technology of Landscape Architecture under State Forestry and Grassland Administration,Yunnan Engineering Research Center for Functional Flower Resources and Industrialization,Kunming 650224;Kunming Greening Engineering Quality Detecting Test Station,Kunming 650224)
出处
《生物技术通报》
CAS
CSCD
北大核心
2024年第8期142-151,共10页
Biotechnology Bulletin
基金
国家“十三五”重点研发计划项目(2019YFD1001005)
云南省万人计划青年拔尖人才项目(YNWR-QNBJ-2019-211)。
关键词
滇山茶
bHLH基因家族
花色
进化树
全长转录组
生物信息学
Camellia reticulata
bHLH gene family
flower color
phylogenetic tree
full-length transcriptome
bioinformatics
作者简介
周麟,男,硕士研究生,研究方向:园林植物资源利用与开发,E-mail:1617574776@qq.com;通讯作者:屈燕,女,教授,研究方向:园林植物资源利用与开发,E-mail:quyan@swfu.edu.cn。
