摘要
目的:明确野鸦椿果实总三萜的最佳提取工艺,并探究野鸦椿果实总三萜对HepG2细胞增殖的抑制作用。方法:以超声提取的方式,总三萜得率为指标,采用单因素试验结合响应面优化法考察野鸦椿果实总三萜最佳提取工艺。采用CCK-8法检测野鸦椿果实总三萜对HepG2细胞增殖抑制作用。结果:影响野鸦椿果实总三萜超声提取的因素顺序为溶剂浓度>提取时间>液料比,最优提取工艺条件为液料比为51∶1(mL·g^(-1)),甲醇浓度69%,提取时间58 min,提取率为(8.49±0.15)%。通过乙酸乙酯萃取和MCI柱色谱纯化后,野鸦椿总三萜纯度可达66.4%。野鸦椿总三萜对HepG2细胞增殖的抑制较好,呈剂量依赖性;IC_(50)为20.302±1.65μg·mL^(-1)。结论:野鸦椿果实总三萜超声提取最优工艺为液料比51∶1(mL·g^(-1)),甲醇浓度69%,超声提取58 min,该方法较为稳定,操作方便快捷。总三萜体外抑制HepG2细胞增殖效果良好。
Objective:To optimize the extraction process of total triterpenes from the fruits of Yeyachun and investigating their inhibition effect of Hep G2 cell proliferation.Methods:The best extraction process of total triterpenes from the fruits of Yeyachun was investigated by ultrasonic extraction with total triterpene yield as an indicator,using a one-way test combined with response surface optimization method.Inhibition of Hep G2 cell proliferation by total triterpenes was tested using CCK-8 assay.Results:The order of factors affecting the ultrasonic extraction of total triterpenes was solvent concentration>extraction time>liquid-material ratio.The optimal extraction conditions were liquid-material ratio of 51:1(mL·g^(-1)),methanol concentration of 69%,and extraction time of 58 min,with an extraction rate of(8.49±0.15)%.After ethyl acetate extraction and purification by MCI column chromatography,the purity of the total triterpenes reached 66.4%.It inhibited Hep G2 cell proliferation in a dosedependent manner.The IC_(50)was 20.302±1.65μg·mL^(-1).Conclusion:The optimal extraction process of total triterpenes from fruits of Yeyachun is liquid-material ratio of 51:1(mL·g^(-1)),methanol concentration of 69%,and ultrasonic extraction of 58 min.This method is relatively stable and convenient and fast.Total triterpenes inhibit HepG2-cell proliferation well in vitro.This study lays the experimental foundation for the development and application of the medicinal components of Yeyachun fruits.
作者
康钦钊
云东
樊馨璐
阿比小龙
尤悦苹
曾佑琴
谭玉柱
陈胡兰
KANG Qinzhao;YUN Dong;FAN Xinlu;A-BI Xiaolong;YOU Yueping;ZENGYouqin;TAN Yuzhu;CHEN Hulan(School of Pharmacy,Chengdu University of Traditional Chinese Medicine,Chengdu 611137,Sichuan;School of Medical Technology,Chengdu University of Traditional Chinese Medicine,Chengdu 611137 Sichuan;State Key Laboratory of Characteristic Chinese Medicine Resources in Southwest China,Chengdu 611137,Sichuan)
出处
《中药与临床》
2024年第3期24-29,共6页
Pharmacy and Clinics of Chinese Materia Medica
基金
重庆市食品药品检验检测研究院(322019047)
成都中医药大学“杏林学者”学科人才科研提升计划-青基人才专项(330022010)。
关键词
野鸦椿
总三萜
提取工艺
抑制HepG2细胞增殖
Yeyachun(Euscaphis japonica(Thunb.)Dippel.)
total triterpenes
extraction process
inhibition of the HepG2 cell proliferation
作者简介
康钦钊(1999-),男,硕士研究生,研究方向:中药有效成分分析应用研究,Tel:15983188271,Email:2677014903@qq.com;通讯作者:谭玉柱(1985-),男,博士,副教授,研究方向:中药资源化学价值发现及综合开发利用,Tel:18683686528,Email:tanyuzhu@cdutcm.edu.cn;通讯作者:陈胡兰(1974-),女,博士,教授,研究方向:中药药效物质基础与中药评价与分析研究,Tel:15828388570,Email:chenhulan@cdutcm.edu.cn。
