摘要
旨在研究内江猪群体的亲缘关系和遗传结构。采用“中芯一号”芯片检测30头内江猪的单核苷酸多态性(SNP);采用GCTA软件构建G矩阵,PLINK软件构建状态同源(IBS)距离矩阵,分析内江猪群体的亲缘关系;采用Mega X软件构建群体进化树,分析内江猪群体的遗传结构。结果:共检测到57466个SNP位点,29468个SNP位点通过质量控制;内江猪试验群体具有中等程度的亲缘关系;群体进化树将内江猪公猪被分为6个血缘,血缘数量与传统系谱记录的基本相同;基于连续性纯合片段(ROH)的平均近交系数为0.243。综上,内江猪血缘较少,有一定程度的近交,可引入新血缘,提高群体遗传多样性和种群质量。
This study was to investigate the genetic relationships and genetic structure in the Neijiang pig population.Single nucleotide polymorphism(SNP)was detected in 30 Neijiang pigs using the SNP bead chip.The GCTA software was used to construct a G matrix,and the PLINK software was employed to construct an identity by state(IBS)distance matrix,in order to analyze the genetic relationships of the Neijiang pig population.The Mega X software was used to construct a population evolution tree and the genetic structure of the Neijiang pig population was analyzed.The results showed that a total of 57466 SNPs loci were detected,and 29468 SNPs loci passed quality control.The experimental Neijiang pig population had a moderate degree of kinship.The population evolution tree divided Neijiang pigs and boars into 6 blood lineages,with the same number as recorded in traditional genealogies.The average inbreeding coefficiency based on ROH was 0.243In summary,Neijiang pigs had relatively a little blood relationship,low genetic diversity and some degree of inbreeding.Therefore,it is necessary to introduce new blood tie to improve the genetic diversity of the population and the population quality.
作者
杨雪梅
陶璇
顾以韧
梁艳
雷云峰
杨跃奎
曾凯
王言
陈晓晖
龚建军
吕学斌
YANG Xuemei;TAO Xuan;GU Yiren;LIANG Yan;LEI Yunfeng;YANG Yuekui;ZENG Kai;WANG Yan;CHEN Xiaohui;GONG Jianjun;LÜXuebin(Sichuan Province Key Laboratory of Animal Breeding and Genetics,Sichuan Academy of Animal Sciences,Chengdu 610066,China;Southwest Minzu University,Chengdu 610041,China)
出处
《畜牧与兽医》
CAS
北大核心
2024年第9期23-27,共5页
Animal Husbandry & Veterinary Medicine
基金
国家重点研发计划项目(2021YFD1301101,2021YFD-1301104)
四川省重大科技专项(2021ZDZX0011,2021ZDZX0008)
四川省重点研发项目(2022YFN0047,2023YFN0088,2023YFN0002)
国家现代农业产业技术体系项目(CARS-35)
现代农业产业技术体系四川创新团队项目(sccxtd-2024-08)
省财政运行专项(SASA2024CZYX001)
天府农业大师项目(He zhiping-2022)。
作者简介
第一作者:杨雪梅,女,硕士,副研究员,研究方向为猪遗传育种与繁殖;通信作者:吕学斌,博士,研究员,研究方向为猪遗传育种与繁殖,E-mail:359818018@qq.com。
