摘要
试验旨在探究在镉污染严重牧区添加外源谷胱甘肽(GSH)对紫花苜蓿的光合作用和抗氧化酶的影响。试验以中苜1号、WL525HQ、甘农3号、甘农9号为试验材料,设置对照组(CK组,Hoagland培养液)、镉处理组(Cd组,Hoagland+15 mg/L Cd)和混合处理组(Cd+GSH组,Hoagland+15 mg/L Cd+25 mg/L GSH)进行水培试验。测定叶片相对含水量(RWC)、叶绿素含量(SPAD)、气孔导度(Gs)、净光合速率(Pn)、胞间CO_(2)浓度(Ci)、蒸腾速率(Tr)以及丙二醛(MDA)含量、脯氨酸(Pro)含量和抗氧化酶活性。结果表明:与Cd组相比,Cd+GSH组各品种紫花苜蓿的RWC、SPAD、Gs、Pn、Ci、Tr均有一定程度的提高。与Cd组相比,Cd+GSH组各品种的MDA含量显著降低(P<0.05),WL525HQ、甘农3号、中苜1号添加外源GSH后Pro含量显著降低(P<0.05)。与Cd组相比,Cd+GSH组各品种过氧化物酶(POD)和抗坏血酸过氧化物酶(APX)活性显著降低(P<0.05),甘农9号和甘农3号超氧化物歧化酶(SOD)活性显著降低(P<0.05),WL525HQ、甘农9号、甘农3号过氧化氢酶(CAT)的活性显著提高(P<0.05)。研究表明,添加外源GSH后可缓解镉污染严重牧区紫花苜蓿受到的镉的毒害作用,降低氧化损伤,提高其适应能力,且紫花苜蓿各生理指标间具有一定的相关关系。
The experiment aimed to explore the effects of adding exogenous glutathione on the photosynthesis and antioxidant enzyme activity of Medicagosativa in severely cadmium(Cd)polluted pasture areas.The materials for the experiment were Zhongmu No.1,WL525HQ,Gannong No.3,and Gannong No.9,with three groups set up:The control group(CK group,Hoagland culture solution),the Cd treatment group(Cd group,Hoagland+15 mg/L Cd),and the combined treatment group(Cd+GSH group,Hoagland+15 mg/L Cd+25 mg/L GSH)for hydroponic experiments.The relative water content of leaves(RWC),chlorophyll content(SPAD),stomatal conductance(Gs),net photosynthetic rate(Pn),intercellular CO_(2)concentration(Ci),transpiration rate(Tr),as well as the content of malondialdehyde(MDA),proline(Pro),and antioxidant enzyme activity were measured.The results showed that compared with the Cd group,the RWC,SPAD,Gs,Pn,Ci,and Tr of each variety in the Cd+GSH group were all improved to some extent.Compared with the Cd group,the MDA content of each variety in the Cd+GSH group was significantly reduced(P<0.05),and the Pro content of WL525HQ,Gannong No.3,and Zhongmu No.1 was significantly reduced after the addition of exogenous GSH(P<0.05).Compared with the Cd group,the activities of peroxidase(POD)and ascorbate peroxidase(APX)in each variety of the Cd+GSH group were significantly reduced(P<0.05),the superoxide dismutase(SOD)activity of Gannong No.9 and Gannong No.3 was significantly reduced(P<0.05),and the activity of catalase(CAT)in WL525HQ,Gannong No.9,and Gannong No.3 was significantly increased(P<0.05).The study indicates that the addition of exogenous GSH can alleviate the toxic effects of Cd on Medicagosativa in severely Cd-polluted pasture areas,reduce oxidative damage,and improve its adaptability,and there is a certain correlation between various physiological indicators of alfalfa.
作者
周德
黄薇
唐卿雁
李博
钱朋
杨姝
ZHOU De;HUANG Wei;TANG Qing-yan;LI Bo;QIAN Peng;YANG Shu
出处
《饲料研究》
CAS
北大核心
2024年第14期75-80,共6页
Feed Research
基金
国家自然科学基金项目(项目编号:31860112)。
关键词
镉胁迫
紫花苜蓿
谷胱甘肽
光合作用:抗氧化酶
cadmium stress
Medicagosativa
glutathione
photosynthesis
antioxidant enzymes
作者简介
第一作者:周德,硕士在读,研究方向为牧草与饲料科学;通信作者:杨姝,博士,副教授,硕士生导师。
