摘要
建立HPLC法测定颍半夏中10种成分的含量,热图聚类分析栽培颍半夏和野生颍半夏10种成分的差异。色谱条件如下:采用资生堂C_(18)色谱柱(4.6 mm×250 mm,5μm);以乙腈(A)-0.01%磷酸溶液(B)为流动相,梯度洗脱;流速1.0 mL/min;双波长检测;柱温30℃;进样量15μL。结果显示,颍半夏中10种成分的分离效果较好,方法学考察符合相关要求,热图聚类结果显示颍半夏具有一定的环境聚类特性。该方法为颍半夏质量评价、栽培环境选择等提供依据。
A dual wavelength method was established of HPLC for the determination of 10 components in Pinelliae Ternata in Yingshang,and the difference of 10 components in cultivated and wild Pinelliae Ternata in Yingshang was analyzed by heat map cluster.The chromatographic conditions are as follows:Shiseido C_(18) column(4.6 mm×250 mm,5μm)was used.Gradient elution was performed with acetonitrile(A)-0.01%phosphoric acid(B)as mobile phase.The flow rate was 1.0 mL/min.Dual wavelength detection was adopted;Column temperature:30℃;the injection volume was 15μL.The result shows that the separation effect of 10 components of Pinelliae Ternata in Yingshang was better.Methodological investigation meets relevant requirements.The results of heat map clustering showed that wild Pinelliae Ternata had certain environmental clustering characteristics,with a higher content of target components in it.The establishment of this method could provide a basis for quality evaluation and cultivation base selection of Pinelliae Ternata in Yingshang.
作者
邢亚东
杨兵
丁琪
罗凯文
XING Yadong;YANG Bing;DING Qi;LUO Kaiwen(School of Pharmacy,Bengbu Medical University,Bengbu 233030,China;School of Pharmacy,Hebei University,Baoding 071002,China)
出处
《宿州学院学报》
2024年第6期33-38,共6页
Journal of Suzhou University
基金
安徽省自然科学项目(1908085QH376)
安徽省中医药传承创新科研项目(2020ccyb20)
安徽省大学生创新创业训练计划项目(S202110367085)
蚌埠医学院自然科学重点项目(2021byzd034)。
关键词
颍半夏
琥珀酸
葫芦巴碱
核苷
HPLC
Pinelliae Ternata in Yingshang
Succinic acid
Trigonelline
Nucleoside
HPLC
作者简介
邢亚东(1987-),安徽阜阳人,硕士,主管药师,研究方向:中药复方质量控制及药效学研究;通信作者:罗凯文(1988-),湖北随州人,博士,副教授,研究方向:中药质量控制及标准。
