摘要
Dear editor,Cas13,a class Ⅱ type Ⅵ CRISPR‒Cas endonuclease with two higher eukaryotes and prokaryotes nucleotide-binding(HEPN)domains,exclusively binds to and cleaves single-stranded RNA by a complementary guide RNA(gRNA)(Makarova et al.,2020).RfxCas13d(also known as CasRx)is an ortholog of CRISPR‒Cas13 with a relatively small size and robust RNA knockdown capability in mammalian cells(Konermann et al.,2018).Owing to its simplicity and high efficiency,CasRx has been applied for RNA targeting in various types of cells and organisms.Nevertheless,most of these studies failed to control the nuclease activity of CasRx spatiotemporally.The re-engineered split-Cas9 system enables inducible genome editing and epigenetic modulation through chemical-induced reassembly of the Cas9 protein(Zetsche et al.,2015).However,tools that allow for efficient and controllable RNA knockdown by a split-Cas13 system remain to be established.
基金
supported by the National Natural Science Foundation of China(82203444 and 82273161)
the Natural Science Foundation of Jiangsu Province(BK20220672)。
作者简介
Correspondence to:Yang Li,contributed equally to thiswork,E-mail:younglee@zju.edu.cn;Qiang Sun,contributed equally to thiswork;Zhi Yang,contributed equally to thiswork;Correspondence to:Gang Yuan,E-mail:gangy2021@wchscu.cn。
