摘要
汉麻花叶用乙醇浸泡提取后的残渣经过水提醇沉后,得到汉麻多糖-I,水相浓缩至小体积用乙酸乙酯萃取后,水层直接减压浓缩得到汉麻多糖-II,乙酸乙酯萃取后的水层继续用正丁醇萃取,减压浓缩得到汉麻多糖-III。用苯酚-硫酸法对3种汉麻多糖进行多糖质量分数的测定,并进行DPPH、ABTS、羟基、超氧阴离子自由基清除实验,结果表明,3种汉麻多糖对DPPH清除率和ABTS清除率都有良好的效果,汉麻多糖-II抗氧化活性最强,清除DPPH、ABTS、羟基、超氧阴离子自由基率的IC50分别为0.012,0.043,0.138,1.857 mg/mL。
Cannabis polysaccharide-I was obtained by water extraction and alcohol precipitation from the residue of cannabis soaked in ethanol.Polysaccharide-I was obtained from the residue through water extraction and alcohol precipitation.The water phase was concentrated and then extracted with ethyl acetate,resulting in the isolation of cannabis polysaccharide-II.After ethyl acetate extraction,the aqueous layer was further processed with n-butanol and concentrated under reduced pressure to yield cannabis polysaccharide-III.The polysaccharide content of the three types of cannabis polysaccharides was determined using the phenol-sulfuric acid method.Additionally,scavenging experiments were conducted to evaluate their antioxidant activity against DPPH,ABTS,hydroxyl,and superoxide anion free radicals.The experimental results demonstrated that the three types of cannabis polysaccharides had good effects on DPPH clearance rate and ABTS clearance rate.Among them,polysaccharide-II showed the strongest antioxidant activity,with IC50 values for scavenging DPPH,ABTS,hydroxyl radical,and superoxide anion radicals at 0.012,0.043,0.138,1.857 mg/mL,respectively.
作者
刘东辉
程亮
赵英楠
孙立秋
王丹
李军
时志春
霍金海
赵明
张树军
LIU Dong-hui;CHENG Liang;ZHAO Ying-nan;SUN Li-qiu;WANG Dan;LI Jun;SHI Zhi-chun;HUO Jin-hai;ZHAO Ming;ZHANG Shu-jun(College of Chemistry and Chemical Engineering,Qiqihar University,Heilongjiang Qiqihar 161006,China;Technology Innovation Center of Industrial Hemp for State Market Regulation,Heilongjiang Qiqihar 161006,China;Heilongjiang Industrial Hemp Processing Technology Innovation Center,Heilongjiang Qiqihar 161006,China;Institute of Chinese Medicine,Heilongjiang Academy of Traditional Chinese Medicine,Heilongjiang Harbin 150036,China)
出处
《齐齐哈尔大学学报（自然科学版）》
2024年第1期51-55,共5页
Journal of Qiqihar University(Natural Science Edition)
基金
黑龙江省省属高等学校基本科研业务费科研项目(145109501)。
关键词
汉麻花叶
多糖
抗氧化
Cannabis flower leaves
polysaccharides
anti-oxidant
作者简介
刘东辉(1999-),男,黑龙江鹤岗人,硕士在读,主要从事天然产物化学研究,1578333130@qq.com;通讯作者:张树军(1964-),男,辽宁凌源人,教授,博士,主要从事天然产物化学研究,chou2035@sina.com。
