摘要
目的 建立五重实时荧光聚合酶链式方法 快速筛查食用淀粉及其制品中植物源性成分。方法 首先设计豌豆、绿豆、木薯、玉米的特异性引物、探针,建立多重实时荧光聚合酶链反应(polymerase chain reaction,PCR)检测方法 ,再验证方法 的特异性和绝对灵敏度,最后通过样品掺假模拟实验验证方法 的检出限。结果 该方法 的特异性强,仅能检出豌豆源性、绿豆源性、木薯源性、玉米源性成分;灵敏度较高,对绿豆源性和木薯源性的检测灵敏度可达到1×10^(–1) ng/μL水平,豌豆源性和玉米源性的检测灵敏度可达到1×10^(–2) ng/μL水平。结论 本研究建立的五重实时荧光PCR方法 可用来区分市场上淀粉及其制品中的豌豆源性、绿豆源性、玉米源性和木薯源性成分,具有较好的应用价值。
Objective To establish a five fold real-time fluorescence polymerase chain reaction(PCR)is developed for rapid screening of plant-derived components in edible starch and its products.Methods Firstly,specific primers and probes were designed for peas,mung beans,cassava,and corn,and a multiplex real-time fluorescence PCR detection method was established.Furthermore,the specificity and absolute sensitivity of the method were verified,and finally,the limit of detection of the method was verified through adulteration simulation experiments.Results The method had strong specificity and couid only detect components derived from peas,mung beans,cassava and corn,the method had high sensitivity,the detection sensitivity for mung bean and cassava was 1×10^(-1) ng/pL,the detection sensitivity of pea and corn was 1×10^(-2)μg/pL.Conclusion The five fold real-time fluorescence PCR method established in this study can be used to distinguish pea derived,mung bean derived,corn derived,and cassava derived components in starch and its products on the market,and it has good practical value.
作者
张明娟
余秋地
王娟
李根容
肖昭竟
周朝旭
ZHANG Ming-Juan;YU Qiu-Di;WANG Juan;LI Gen-Rong;XIAO Zhao-Jing;ZHOU Zhao-Xu(Chongging Academy of Metrology and Quality Inspection,Chongqing 401120,China)
出处
《食品安全质量检测学报》
CAS
2024年第13期207-214,共8页
Journal of Food Safety and Quality
基金
重庆市市场监督管理局科研计划项目(CQSJKJ2022036)。
关键词
五重实时荧光聚台酶链式反应
淀粉
淀粉制品
检测方法
five fold real-time fluorescence polymerase chain reaction
starch
starch products
detection method
作者简介
张明娟,硕士研究生,高级工程师,主要研究方向为食品微生物检测和分子生物学检测。E-mail:467613678@qq.com;通信作者:周朝旭,高级工程师,主要研究方向为食品安全检测。E-mail:4921671@qq.com。
