摘要
从高抗白粉病的南瓜植株内分离筛选出对南瓜白粉病有较好防治效果的内生拮抗细菌菌株,为南瓜白粉病的生物防治提供新途径。采用孢子萌发抑制法、叶盘漂浮法和温室盆栽试验筛选出有较好防效的内生细菌,用形态观察、生理生化测定和分子生物学方法进行鉴定。结果表明,从高抗白粉病的南瓜自交系22G3植株内分离获得29株内生拮抗细菌菌株,5个菌株对南瓜白粉菌的孢子萌发抑制率在50%以上,其中菌株B4-2对白粉菌的孢子萌发抑制率最高,为88.65%。叶盘和室内盆栽试验结果均表明,菌株B4-2对南瓜白粉病的防治效果最好,防治效果分别达79.58%和83.21%。不同稀释倍数的B4-2发酵液对南瓜白粉病均有一定的防治效果,随着稀释倍数的增大,防治效果降低,其中菌株B4-2发酵液原液对南瓜白粉病的防治效果最好,达82.14%。通过形态特征、生理生化特征及基因序列分析,鉴定菌株B4-2为蜡样芽孢杆菌(Bacillus cereus)。综上所述,蜡样芽孢杆菌B4-2能较好地防治南瓜白粉病,有一定的开发潜力。
In order to find the new biological control method against pumpkin powdery mildew,this experiment screened the endophytic antagonistic bacteria against pumpkin powdery mildew from high resistance plants.The spore germination inhibition method,leaf disk floating method and greenhouse pot experiment method were used to select endophytic bacteria with good control effect,which was identified by morphological observation,physiological and biochemical determination as well as molecular biology method.The results showed that 29 endophytic antagonistic bacterial strains were isolated from the high resistant pumpkin inbred line 22G3.Among them,5 strains had an inhibition rate of over 50%on the spore germination of powdery mildew,and B4-2 had the highest inhibition rate of 88.65%.Both leaf disc and potting experiments showed that the strain B4-2 had the best control effect on pumpkin powdery mildew,with the control effects of 79.58%and 83.21%,respectively.All the different dilution ratios of B4-2 fermentation had certain control effects on pumpkin powdery mildew.As the dilution ratio increased,the prevention effect decreased.The prevention effect of original B4-2 solution was the best and was 82.14%.B4-2 was identified as Bacillus cereus by morphological,physiological,biochemical and gene sequence analysis methods.In summary,B.cereus B4-2 can effectively control pumpkin powdery mildew,which has certain potential for development.
作者
李亚莉
齐立本
LI Yali;QI Liben(Vegetable Institute,Gansu Academy of Agricultural Sciences,Lanzhou 730070,Gansu,China)
出处
《中国瓜菜》
CAS
北大核心
2024年第8期51-56,共6页
China Cucurbits And Vegetables
基金
甘肃省农业科学院科研条件建设及成果转化项目(2022GAAS33)
国家重点研发计划项目(2022YFD1602110)。
关键词
南瓜
白粉病
蜡样芽孢杆菌
生物防治
防治效果
Pumpkin
Powdery mildew
Bacillus cereus
Biological control
Control efficiency
作者简介
李亚莉,女,副研究员,主要从事南瓜栽培与育种工作。E-mail:yaligsau@163.com。
