摘要
黄瓜(Cucumis sativus)是中国设施栽培的主要蔬菜作物之一。高温会造成黄瓜生长受阻、抗性降低,导致产量和品质下降。本研究采用同源克隆方法,从黄瓜叶片的cDNA中克隆了一个WRKY转录因子基因CsWRKY20。生物信息学分析结果表明,CsWRKY20基因的CDS长1 602 bp,共编码533个氨基酸;CsWRKY20蛋白分子量为132 959.43 Da,等电点为4.97,定位于细胞核,含有两个典型的保守WRKY结构域,与甜瓜CmWRKY20蛋白的亲缘关系最近。qRT-PCR检测CsWRKY20基因在黄瓜不同器官中的表达情况,发现其在营养器官和生殖器官中均有表达,在果刺中表达量最高。五叶期黄瓜幼苗受42℃高温胁迫1 h后,CsWRKY20的表达水平显著升高,6 h后相对表达量恢复到初始水平。本研究结果可为黄瓜耐高温品种选育提供一定的理论基础。
Cucumber is one of the major vegetables in facility greenhouse in China.High temperature can cause cucumber growth to be stunted and resistance to be reduced,resulting in yield and quality decline.In this study,a WRKY transcription factor,CsWRKY20,was cloned from the cDNA of cucumber leaves using homologous cloning technology.The CDS length of the CsWRKY20 was 1602 bp,encoding 533 amino acids.The molecular weight of the CsWRKY20 protein was 132959.43 Da,and the isoelectric point was 4.97.The CsWRKY20 was predicted to be localized in nucleus,contained two typical conserved WRKY domains,and had the closest sequence homology to CmWRKY20 of muskmelon.The real-time quantitative PCR analysis showed that CsWRKY20 was expressed in both nutrient and reproductive organs,with the highest expression level in cucumber fruit spines.High temperature stress resulted in significant increase of CsWRKY20 expression level at 1 hour,and then decreased and recovered to the initial expression level after 6 hours.The results of this study could provide a theoretical basis for breeding high temperature-tolerant variety of cucumber.
作者
陈岳
鲍文敏
徐俐琴
张微微
Chen Yue;Bao Wenmin;Xu Liqin;Zhang Weiwei(Shanghai Vocational College of Agriculture and Forestry,Shanghai 201699,China)
出处
《山东农业科学》
北大核心
2024年第7期32-38,共7页
Shandong Agricultural Sciences
基金
上海市“科技创新行动计划”农业领域项目(20392001300)
上农植慧种质资源创新团队项目(KY6-0100-24-01)。
关键词
黄瓜
WRKY转录因子
生物信息学分析
高温胁迫
基因表达分析
Cucumber
WRKY transcription factor
Bioinformatics analysis
High temperature stress
Gene expression analysis
作者简介
陈岳(1991-),男,博士,主要从事黄瓜基因克隆与育种研究。E-mail:yuechen321@aliyun.com;通信作者:张微微(1981-),女,博士,教授,主要从事植物栽培与育种研究。E-mail:zhangww@shafc.edu.cn。
