摘要
目的分析视网膜母细胞瘤(Rb)患儿血清长链非编码RNA核内富集转录物1(LncRNA NEAT1)、三叶因子1(TFF1)表达及预后评估价值。方法选取2018年1月—2021年1月首都医科大学附属北京安贞医院南充医院眼科手术治疗的Rb患儿85例作为Rb组,以同期体检健康者60例为健康对照组。采用实时荧光定量PCR检测血清LncRNA NEAT1水平,采用酶联免疫吸附试验检测血清TFF1;分析血清LncRNA NEAT1、TFF1与临床病理特征的关系;Cox回归分析Rb预后影响因素;受试者工作特征(ROC)曲线分析血清LncRNA NEAT1、TFF1对Rb患儿预后的评估价值。结果Rb组血清LncRNA NEAT1水平高于健康对照组,血清TFF1低于健康对照组(t/P=38.305/<0.001、34.858/<0.001)。与肿瘤直径<20 mm、病理分化型Rb患儿比较,肿瘤直径≥20 mm、未分化型Rb患儿血清LncRNA NEAT1较高,TFF1较低(LncRNA NEAT1:t/P=17.925/<0.001,16.848/<0.001;TFF1:t/P=12.505/<0.001,8.120/<0.001)。血清LncRNA NEAT1高表达组3年无进展生存率低于低表达组(57.50%vs.88.89%),TFF1低表达组3年无进展生存率低于高表达组(57.14%vs.90.70%)(Log rankχ^(2)/P=13.551/<0.001、15.310/<0.001)。病理未分化型、血清LncRNA NEAT1升高是影响Rb预后的危险因素,血清TFF1升高是其保护因素[HR(95%CI)=1.523(1.147~2.024),1.473(1.108~1.957),0.612(0.413~0.908)];血清LncRNA NEAT1、TFF1及二者联合预测Rb预后的曲线下面积分别为0.836、0.861、0.921,二者联合大于血清LncRNA NEAT1、TFF1单项检测(Z=4.823、4.312,P均<0.001)。结论Rb患儿血清LncRNA NEAT1升高,血清TFF1水平降低,两者与肿瘤最大径及病理分型有关,均是影响Rb患儿预后的因素,两者联合有助于评估患者预后。
Objective To investigate the expression and prognostic value of serum long chain non coding RNA nuclear enriched transcript 1(LncRNA NEAT1)and trefoil factor 1(TFF1)in children with retinoblastoma(Rb).Methods Eighty-five Rb patients who underwent ophthalmic surgery at Ophthalmology Department of Beijing Anzhen Hospital Affiliated to Capital Medical University Nanchong Hospital from January 2018 to January 2021 were selected as the Rb group,while 60 healthy individuals who underwent physical examinations during the same period were selected as the healthy control group.Real time fluorescence quantitative PCR was used to detect serum LncRNA NEAT1 levels,and enzyme-linked immunosorbent assay was used to detect serum TFF1 levels;the relationship between serum LncRNA NEAT1,TFF1 and clinical pathological characteristics was analyzed;the evaluation value of serum LncRNA NEAT1 and TFF1 in Rb patients was analyzed by job characteristic curve of subjects.Results The serum LncRNA NEAT1 in the Rb group was(3.12±0.43)higher than that in the healthy control group(0.82±0.21),the serum TFF1 of the Rb group was(22.14±4.61)μg/L,which was lower than that of the healthy control group(64.01±9.63)μg/L(t/P=38.305/<0.001,34.858/<0.001).Compared with patients with tumor diameter<20 mm and pathologically differentiated Rb,patients with tumor diameter≥20 mm and undifferentiated Rb had higher serum LncRNA NEAT1 and lower TFF1(Lnc RNANEAT1:t/P=17.925/<0.001,16.848/<0.001;TFF1:t/P=12.505/<0.001,8.120/<0.001).The 3-year progression free survival rate of the high expression group of serum LncRNA NEAT1 was lower than that of the low expression group(57.50%vs.88.89%),and the 3-year progression free survival rate of the low expression group of TFF1 was lower than that of the high expression group(57.14%vs.90.70%)(Log rankχ^(2)/P=13.551/<0.001,15.310/<0.001);Pathologically undifferentiated type and high serum LncRNA NEAT1 were risk factors affecting the prognosis of Rb,while high serum TFF1 was a protective factor[HR(95%)CI=1.523(1.147-2.024),1.473(1.108-1.957),0.612(0.413-0.908)];The area under the curve of serum LncRNA NEAT1,TFF1,and their combined prediction of Rb prognosis were 0.836,0.861,and 0.921,respectively,which were larger than the single detection of serum LncRNA NEAT1 and TFF1(Z=4.823,4.312,P<0.001).Conclusion Serum LncRNA NEAT1 levels increase and serum TFF1 levels decrease in Rb patients,both of which are related to the maximum tumor diameter and pathological classification,and are factors affecting the prognosis of Rb patients.The combination of the two can help evaluate the prognosis of patients.
作者
万宇
邹云春
王岚
李娜
何艺岚
王淑
Wan Yu;Zou Yunchun;Wang Lan;Li Na;He Yilan;Wang Shu(Department of Ophthalmology,Beijing Anzhen Hospital Affiliated to Capital Medical University Nanchong Hospital,Sichuan Province,Nanchong 637000,China;不详)
出处
《疑难病杂志》
CAS
2024年第8期987-992,共6页
Chinese Journal of Difficult and Complicated Cases
基金
四川省卫生健康科研课题普及项目(19PJ224)。
作者简介
通信作者:王岚,E-mail:187195689@qq.com。
