摘要
目的:筛选适合分离纯化秦艽中龙胆苦苷的大孔树脂并确定纯化工艺参数。方法:以大孔树脂对龙胆苦苷的吸附量和解析率为指标,选择最佳的树脂进行富集。采用单因素实验法对上样浓度、醇洗脱浓度、吸附时间、洗脱体积和流速进行考察,从而确定最佳纯化工艺。结果:LX-70型大孔树脂的吸附和解析效果最佳,其动态饱和吸附量为45.41 mg/g,最佳工艺参数为:龙胆苦苷上样液的质量浓度为30.20 mg/mL,吸附1.5 h,洗脱剂为30%乙醇,乙醇洗脱4B V,洗脱流速1~1.5 BV/h,龙胆苦苷的纯度可达到66.54%,龙胆苦苷的转移率大于95%。结论:LX-70型树脂对龙胆苦苷有较高的吸附量和解析率,洗脱溶剂的用量也更少,其工艺参数可供放大生产参考。
Objective:To screen the suitable macroporous resins for the isolation and purification of gentianoside from Gentiana macrophylla Pall.and to determine the purification process parameters.Methods:The adsorption amount and resolution rate of gentianoside by the macroporous resin were used as indexes to select the best resin for enrichment.The upper sample concentration,alcohol elution concentration,adsorption time,elution volume,and flow rate were examined by a one-way experimental method,to determine the optimal purification process.Results:The LX-70 macroporous resin showed the best adsorption and analysis effect,its dynamic saturation adsorption capacity was 45.41mg/g,and the best process parameters were:the mass concentration of the upper sample solution of gentianoside was 30.20mg/mL,the adsorption was performed for 1.5 h,the eluent was 30%ethanol,the ethanol elution was performed at 4BV,the elution flow rate was 1-1.5BV/h,the purity of gentianaside could reach 66.54%,and the transfer rate of gentianaside was more than 95%.Conclusion:The LX-70 resin has a higher adsorption capacity and resolution rate for gentiopicroside,and the amount of elution solvent is less,and its process parameters can be used as a reference for scale-up production.
作者
谭文君
宁夏
刘华石
耿晨阳
沈炜坤
王伟明
Tan Wenjun;Ning Xia;Liu Huashi;Geng Chenyang;Shen Weikun;Wang Weiming(Heilongjiang Academy of Chinese Medicine,Harbin 150036,China;Shenyang PharmaceuticalUniversity,Shenyang 110016,China)
出处
《亚太传统医药》
2024年第7期81-86,共6页
Asia-Pacific Traditional Medicine
关键词
秦艽
龙胆苦苷
大孔吸附树脂
分离纯化
Gentiana Macrophylla Pall.
Gentiopicroside
Macroporous Resin
Separation and Purification
作者简介
谭文君(1998-),男,黑龙江省中医药科学院硕士研究生,研究方向为中药分离纯化及制剂;通讯作者:王伟明(1966-),女,黑龙江省中医药科学院研究员,博士生导师,研究方向为中医药学。E-mail:zyyjy@163.com。
