摘要
目的:建立蜜糠炒白术的高效液相色谱(HPLC)指纹图谱及多成分含量测定方法。方法:采用Agilent Eclipse XDB-C_(18)色谱柱(4.6 mm×250 mm,5μm),乙腈-0.1%磷酸水溶液为流动相,流速为0.8 mL·min^(-1)进行梯度洗脱,柱温为30℃,检测波长为240 nm,建立15批蜜糠炒白术的HPLC指纹图谱,并同时建立白术内酯Ⅰ、Ⅱ、Ⅲ及苍术酮的含量测定方法。结果:15批蜜糠炒白术的指纹图谱呈现24个共有峰,相似度均>0.891;对其中4个共有峰进行指认,分别是白术内酯Ⅲ(11号峰)、白术内酯Ⅱ(16号峰)、白术内酯Ⅰ(20号峰)、苍术酮(24号峰)。主成分分析(PCA)和正交偏最小二乘分析(OPLS-DA)结果均显示,不同产地、同产地不同批次药材制备的蜜糠炒白术质量有着明显的区分;变量重要投影系数(VIP)值>1的共有峰有7个,从大到小分别为1号、14号、4号、24号(苍术酮)、15号、16号(白术内酯Ⅱ)及2号峰。15批蜜糠炒白术的白术内酯Ⅰ、Ⅱ、Ⅲ及苍术酮的含量依次为144.84~642.59μg·g^(-1)、57.67~1086.15μg·g^(-1)、142.45~707.19μg·g^(-1)、968.73~2887.70μg·g^(-1)。结论:建立的蜜糠炒白术指纹图谱及多成分定量分析方法操作简单、稳定可靠,结合化学计量学方法可实现对蜜糠炒白术的质量分析,为蜜糠炒白术质量评价标准的建立提供参考。
Objective:To establish a high-performance liquid chromatography(HPLC)fingerprint and multi-component determination method for stir-fried with honey bran(AMR-HB).Methods:HPLC analysis was performed on the Agilent Eclipse XDB-C_(18)column(4.6 mm×250 mm,5μm).The acetonitrile-0.1%phosphoric acid aqueous solution was set as a mobile phase and the flow rate was 0.8 mL/min for gradient elution.The column temperature was maintained at 30℃and the detection wavelength was 240 nm.The HPLC fingerprint of 15 batches of AMR-HB was established.A method for the determination of atractylodesⅠ,Ⅱ,Ⅲand atractylodes was established simultaneously.Results:There were 24 common peaks in the fingerprints of 15 batches of AMR-HB,and the similarity was greater than 0.891.Four of them were identified as atractylodes lactoneⅢ(peak 11),atractylodes lactoneⅡ(peak 16),atractylodes lactone I(peak 20)and atractylodes ketone(peak 24).The results of principal component analysis(PCA)and orthogonal partial least squares analysis(OPLS-DA)showed that the quality of AMR-HB processed from the AMR of different origins and different batches from the same origin had obvious differences.Seven common peaks with variable importance projection coefficient(VIP)value greater than 1,which were peak 1,peak 14,peak 4,peak 24(Atractylodes ketone),peak 15,peak 16(AtractylideneⅡ)and peak 2 from large to small.The contents of atractylodes lactoneⅠ,Ⅱ,Ⅲand atractylodes ketone in 15 batches of AMR-HB were 144.84-642.59μg/g,57.67-1086.15μg/g,142.45-707.19μg/g and 968.73-2887.70μg/g respectively.Conclusion:The fingerprints and multi-component quantitative analysis methods established in this study are simple,stable and reliable.Combined with the chemometrics method,the quality analysis of AMR-HB can be realized,and it can provide a reference for the establishment of the quality evaluation standard of AMR-HB.
作者
柯瑞
张江山
甘春梅
肖小林
曾鹏辉
张金莲
叶明磊
刘明贵
徐葱茏
杨焕林
刘英
Ke Rui;Zhang Jiangshan;Gan Chunmei;Xiao Xiaolin;Zeng Penghui;Zhang Jinlian;Ye Minglei;Liu Minggui;Xu Conglong;Yang Huanlin;Liu Ying(School of Clinical Medicine,Jiangxi University of Chinese Medicine,Nanchang 330004,China;Jiangxi Jingde Traditional Chinese Medicine Co.,Ltd.,Leping 333302,China;School of Pharmacy,Jiangxi University of Chinese Medicine,Nanchang 330004,China;The Hospital Affiliated to Jiangxi University of Chinese Medicine,Nanchang 330006,China)
出处
《亚太传统医药》
2024年第7期50-56,共7页
Asia-Pacific Traditional Medicine
基金
江西省中医药标准化委员会2021年第一批标准化项目(2020A09)
江西省“双千计划”项目(S2019CXTD2300)。
关键词
蜜糠炒白术
质量评价
指纹图谱
含量测定
建昌帮
产地
Atractylodis Macrocephalae Rhizoma Stir-Fried with Honey Bran
Quality Evaluation
Fingerprint
Content Assay
Jianchang Sect
Habitat
作者简介
柯瑞(1987-),女,江西中医药大学硕士研究生,研究方向为中医儿科学;通讯作者:刘英(1977-),女,江西中医药大学附属医院副主任中药师,研究方向为中医儿科学。E-mail:595317906@qq.com。
