摘要
目的 研究红景天苷(Salidroside,Sal)对人血小板活化聚集的影响以及对人血小板与乳腺癌MDA-MB-468细胞相互作用的影响。方法 采集人全血并制备血小板悬液,采用不同浓度Sal处理后通过血栓弹力图(thromboelastogram,TEG)、流式细胞术检测Sal对血小板活化、聚集的影响。体外培养乳腺癌MDA-MB-468细胞,采用不同浓度Sal处理人血小板,使用凝血酶激活血小板,通过黏附实验和划痕实验分析Sal对血小板与MDA-MB-468细胞相互作用的影响。结果 TEG检测:空白对照组的ADP抑制率为(10.97±12.69)%,各浓度Sal干预组ADP抑制率均高于空白对照组(P<0.05);空白对照组的花生四烯酸(arachidonic acid,AA)抑制率为(8.11±7.84)%,5μmol/L Sal干预组为(25.96±15.18)%,2者比较有差异(P<0.05)。流式细胞术检测:40、60μmol/L Sal组血小板表面CD62P表达分别为(56.5±0.17)%和(65.50±0.36)%,与阳性对照组(76.53±0.49)%比较有差异(P<0.05);40、60μmol/L Sal组血小板表面PAC-1的表达分别为(62.20±0.10)%、(58.47±0.15)%,与阳性对照组(72.10±0.20)%比较有差异(P<0.05)。黏附实验:血小板可与MDA-MB-468细胞产生黏附作用,且活化血小板黏附能力更强。而Sal各组的黏附率较阳性对照组明显降低,与Sal浓度呈负相关。划痕实验:阳性对照组24 h细胞迁移率为(12.71±0.70)%,各浓度Sal处理组细胞迁移率分别为(4.51±0.44)%、(3.85±0.11)%、(5.37±0.36)%、(4.15±0.13)%、(3.55±0.38)%,与阳性对照组比较均降低(P<0.05)。Sal干预48 h后,10、20、40、60μmol/L Sal组细胞迁移率均降低,与阳性对照组比较有差异(P<0.05)。结论 Sal能够抑制凝血酶介导的活化血小板与MDA-MB-468细胞间的黏附及MDA-MB-468细胞的迁移。
Objective To study the effect of Salidroside(Sal) on platelet activation and aggregation and the interaction between human platelets and MDA-MB-468 cells of breast cancer.Methods Human platelets were collected,platelet suspension was prepared,and platelets were treated with different concentrations of Sal.The effects of Sal on platelet activation and aggregation were detected by thromboelastogram(TEG) and flow cytometry.Breast cancer MDA-MB-468 cells were cultured in vitro,and human platelets were treated with different concentrations of Sal,and then activated by thrombin.The effects of Sal on the interaction between platelets and MDA-MB-468 cells were analyzed by adhesion test and scratch test.Results TEG detection:The ADP inhibition rate in the blank control group was(10.97±12.69)%,and the ADP inhibition rate in all Sal intervention groups was higher than that in the blank control group(P<0.05).The AA inhibition rate was(8.11±7.84)% in the control group and(25.96±15.18) % in the 5 μmol/L Sal intervention group,and the difference was statistically significant(P<0.05).Flow cytometry:The expression of CD62P on platelet surface in 40 and 60 μmol/L Sal groups was(56.5±0.17)% and(65.50±0.36)%,respectively,and the difference was statistically significant compared with the positive control group(76.53±0.49)%(P<0.05).The percentages of PAC-1 expression on platelet surface in 40 and 60 μmol/L Sal groups were(62.20±0.10)% and(58.47±0.15)%,and the difference was statistically significant compared with the positive control group(72.10±0.20) %(P<0.05).Adhesion experiment:Platelets can have adhesion with MDA-MB-468 cells,and activated platelets have stronger adhesion ability.The adhesion rate in the Sal group was significantly lower than that in the positive control group,and was negatively correlated with the concentration of Sal.Scratch test:The cell mobility at 24 h in the positive control group was(12.71±0.70)%,and the cell mobility in each Sal treatment group was(4.51±0.44)%,(3.85±0.11)%,(5.37±0.36)%,(4.15±0.13)% and(3.55±0.38)%,respectively,showing significant decrease compared with the positive control group(P<0.05).After 48 h of Sal treatment,the cell mobility of 10,20,40 and 60μmol/L Sal groups decreased,and there was a statistical difference compared with the positive control group(P<0.05).Conclusion Sal can inhibit the adhesion between activated platelets mediated by thrombin and MDA-MB-468 cells and the migration of MDA-MB-468 cells.
作者
邵意涵
邵小宝
朱维娜
陈鑫
周琳
朱培元
SHAO Yihan;SHAO Xiaobao;ZHU Weina;CHEN Xin;ZHOU Lin;ZHU Peiyuan(Nanjing Hospital of Chinese Medicine Affiliated to Nanjing University of Chinese Medicine;Taizhou Women and Children′s Hospital of Wenzhou Medical University,Nanjing 210022,China)
出处
《中国输血杂志》
CAS
2024年第7期754-760,共7页
Chinese Journal of Blood Transfusion
基金
南京市卫生科技发展专项资金项目(YKK21195)。
关键词
红景天苷
血小板
乳腺癌
活化
迁移
Salidroside(Sal)
platelet
breast cancer
activation
migration
作者简介
第一作者:邵意涵(1996-),女,住院医师,Email:1096204069@qq.com;通信作者:朱培元,Email:zhupy@njucm.edu.cn。
