摘要
为探明健康与感病“红阳”猕猴桃根际土壤的细菌种群差异,从湘西州保靖县猕猴桃种植区采集健康与感病2种根际土壤样品,通过纯培养法分离细菌,基于细菌菌落形态特征和16S rRNA基因序列的系统发育分析进行种属鉴定。从2种根际土壤样品中共分离获得30株细菌,经鉴定分属于链霉菌属、红球菌属、芽孢杆菌属、北里孢菌属和节杆菌属5个属;健康猕猴桃根际土壤中优势菌群为芽孢杆菌属和链霉菌属;感病猕猴桃根际土壤中主要是芽孢杆菌属细菌,且没有分离到北里孢菌属细菌。这表明健康与感病“红阳”猕猴桃根际土壤中细菌在种属水平存在差异,推测链霉菌属细菌中可能存在拮抗溃疡病的菌株。
This study aims to reveal the differences of rhizosphere soil microorganisms between the healthy and diseased plants of the kiwifruit variety'Hongyang'.Rhizosphere soil samples of the healthy and diseased plants were collected from the kiwifruit planting area of Baojing County,Xiangxi Prefecture,and bacteria were isolated by the pure culture method.The isolates were identified by morphological observation of colonies and phylogenetic analysis based on 16S rRNA gene sequences.A total of 30 bacterial strains were isolated from two kinds of soil samples,and they were identified as Streptomyces,Rhodococcus,Bacillus,Kitasatospora,and Arthrobacter.Bacillus and Streptomyces were the dominant bacteria in the rhizosphere soil of healthy kiwifruit plants.Bacillus was dominant and Kitasatospora was not discovered in the rhizosphere soil of diseased kiwifruit plants.The results demonstrated differences of bacteria genera in the rhizosphere soil between healthy and diseased plants of the kiwifruit variety'Hongyang',which indicated that there might be antagonistic strains of Streptomyces against kiwifruit canker.
作者
瞿昕怡
彭清忠
向小奇
易浪波
QU Xin-yi;PENG Qing-zhong;XIANG Xiao-qi;YI Lang-bo(College of Biology and Environmental Sciences,Jishou University,Jishou 416000,PRC)
出处
《湖南农业科学》
2024年第6期5-9,共5页
Hunan Agricultural Sciences
基金
湖南省重点研发计划(2022NK2055)
湖南省大学生创新训练计划项目(S202310531067)。
关键词
猕猴桃
根际土壤
可培养细菌
分离
鉴定
kiwifruit
rhizosphere soil
culturable bacteria
isolation
identification
作者简介
瞿昕怡(2002-),女,湖南株洲市人,本科生,研究方向为微生物生态学;通信作者:易浪波。
