摘要
用1、5、10 mg/g阿魏酸添饲草地贪夜蛾初孵幼虫,观察记录幼虫生活历期,测量幼虫体长、虫体质量,统计化蛹率和羽化率。采用转录组测序结合RNA干扰探究草地贪夜蛾解毒代谢酶对阿魏酸的解毒功能。结果表明:与添饲丙酮和清水对照相比,10mg/g阿魏酸处理的幼虫发育历期延长了1.6倍;蛹质量仅为对照的34%;化蛹率和羽化率分别降低了80.4%和77.7%;阿魏酸处理激活草地贪夜蛾多个解毒代谢酶基因表达,沉默解毒酶基因细胞色素氧化还原酶基因SfCYP6B6、甘肽尿苷二磷酸葡萄糖醛酸转移酶基因(SfUGT2B15)可显著增强阿魏酸对草地贪夜蛾的致死效果;与添饲丙酮对照相比,阿魏酸对沉默SfCYP6B6和SfUGT2B15的草地贪夜蛾幼虫的72 h致死率分别提高了36.7%和52.6%。
In this study,1,5,and 10 mg/g ferulic acid were added to the diet of the Spodoptera frugiperda(S.frugiperda)to observe their effects on the larval duration,larval body length,body mass,pupation rate,and emergence rate.Transcriptome sequencing combined with RNA interference was used to investigate the detoxification function of detoxification metabolic enzymes in the S.frugiperda against ferulic acid.The results showed that,compared with the acetone-fed control,the developmental period of larvae treated with 10 mg/g ferulic acid was extended by 1.6 times;the pupal mass was only 34%of the control;and the pupation and eclosion rates were reduced by 80.4%and 77.7%,respectively.Ferulic acid treatment activated the expression of multiple detoxification metabolic enzyme genes in the S.frugiperda,and silencing of detoxification enzymes SfCYP6B6 and SfUGT2B15 could significantly enhance the lethal effect of ferulic acid on the S.frugiperda.Compared with the acetone-fed control,the 72 h lethality rate of ferulic acid on larvae with silenced SfCYP6B6 and SfUGT2B15 increased by 36.7%and 52.6%,respectively.
作者
史智英
贺华良
王建田
陈杰
林宇峰
李有志
SHI Zhiying;HE Hualiang;WANG Jiantian;CHEN Jie;LIN Yufeng;LI Youzhi(College of Plant Protection,Hunan Agricultural University,Changsha,Hunan 410128,China;National Research Center of Engineering Technology for Utilization of Botanical Functional Ingredients,Changsha,Hunan 410128,China;Longhui County Agricultural Bureau of Hunan Province,Longhui,Hunan 422299,China;Dingcheng District Agricultural Bureau of Changde City,Changde,Hunan 415009,China;Agriculture and Rural Department of Hunan Province,Plant Protection and Inspection Station,Changsha,Hunan 410005,China)
出处
《湖南农业大学学报（自然科学版）》
CAS
CSCD
北大核心
2024年第3期62-68,共7页
Journal of Hunan Agricultural University(Natural Sciences)
基金
湖南省重点研发计划项目(2020NK2034)
长沙市自然科学基金项目(kq2202226)。
关键词
阿魏酸
草地贪夜蛾
解毒机制
转录组
解毒代谢酶
ferulic acid
Spodoptera frugiperda
detoxification mechanism
transcriptome
detoxification metabolic enzyme
作者简介
史智英(1998-),女,甘肃平凉人,硕士研究生,主要从事农业昆虫害虫防治研究,1214431790@qq.com;通信作者:李有志,博士,教授,主要从事水稻害虫综合防控研究,liyouzhi@hunau.edu.cn。
