摘要
目的 探讨不同造模方法对皮肤损伤的影响及不同时间段创面皮肤愈合的病理学评价。方法 实验选用SD大鼠78只,雌雄各半,分为正常对照组(14只)、烧伤模型组(24只)、烫伤模型组(24只)和全切皮肤模型组(16只)。其中烫伤模型组采用台式超级控温烫伤仪烫伤皮肤组织,并于创面滴入混合菌,建立浅II度烫伤合并伤口感染模型。烧伤模型组采用自制烧伤锥形瓶烧烫伤皮肤组织,并于创面滴入混合菌,建立浅II度烧伤合并伤口感染模型。全切皮肤模型组采用无菌手术刀对皮肤进行面积为3 cm×3 cm切除,切除不伤及肌肉,建立皮肤全切模型。正常对照组分别于D1、3、4、5、7、10、14取2只动物进行解剖取材,烧伤和烫伤+细菌感染模型组分别于D1、3、5、7、10、14各取4只动物进行解剖取材,全切皮肤模型于D4、7、10、14各取4只动物进行解剖取材,对创面皮肤组织进行病理学检查。结果 大体观察发现,不同模型造模初期皮肤均出现不同程度创面损伤;中期皮肤损伤部位周围均出现红肿、水泡等局部反应;末期皮肤愈合较为完整。显微镜观察发现,各模型初中期皮肤主要表现为表皮变性坏死,角化不全,颗粒层、棘细胞层及基底细胞层缺乏,真皮不同程度炎性细胞浸润;末期皮肤表皮各层结构较为完整,真皮层可见大量新生血管,成纤维细胞聚集及少量炎症细胞浸润。结论 通过对造模不同阶段皮肤组织的病理学评价,较为全面的分析了不同造模方法对皮肤的损伤与愈合情况,为后续药物药效学研究奠定了基础。
Objective To investigate the effects of different method of modeling on skin injury and the pathological evaluation of wound healing in different time periods.Method Seventy-eight SD rats,half male and half female,were divided into normal control group(14 rats),burn model group(24 rats),scald model group(24 rats)and total skin resection group(16 rats).In the scald model group,the skin tissue was scalded by desktop super temperature control scald instrument,and the mixed bacteria were dripped into the wound surface to establish the model of superficial II degree scald combined with wound infection.Burn model group was established by using self-made burn conical flask to burn skin tissue and dripping mixed bacteria into the wound surface to establish the model of superficial II degree burn combined with wound infection.In the total skin resection model group,the skin was resected with a sterile scalpel in an area of 3 cm×3 cm without muscle injury to establish the total skin resection model.Two animals of the normal control group were dissected and the samples were taken on the day 1,3,4,5,7,10 and 14 respectively.Four animals of the burn and scald+bacterial infection model group were dissected and the samples were taken on the day 1,3,5,7,10 and 14 respectively.Four animals of the total skin resection model were dissected respectively on the day 4,7,10 and 14.The skin tissues of the wound were examined pathologically.Result The gross observation showed that the skin of different models had different degrees of wound damage at the initial stage of modeling;in the middle stage,local reactions such as redness,swelling and blisters appeared around the skin injury site;the final skin healing is relatively complete.Microscopic observation showed that the skin of each model in junior middle school was mainly characterized by epidermal degeneration and necrosis,incomplete keratosis,lack of granular layer,acanthocyte layer and basal cell layer,and different degrees of inflammatory cell infiltration in the dermis.At the end of the period,the structure of each layer of skin epidermis is relatively complete,and a large number of new blood vessels,fibroblasts and a small amount of inflammatory cells can be seen in the dermis.Conclusion Through the pathological evaluation of skin tissue at different stages of modeling,the injury and healing of skin caused by different modeling method were comprehensively analyzed,which laid the foundation for the follow-up pharmacodynamic study.
作者
肖洒
孙军
彭冬冬
唐海亮
姜德建
张妙红
XIAO Sa;SUN Jun;PENG Dongdong;TANG Hailiang;JIANG Dejian;ZHANG Miaohong(Hunan Prima Drug Research Center Co.Ltd.,Hunan Key Laboratory of Phamacodynamics and Safety Evaluation of New Drugs,Changsha 410329,China)
出处
《实验动物科学》
2024年第3期18-25,共8页
Laboratory Animal Science
基金
湖南省企业科技创新创业团队支持计划(2021)。
关键词
浅II度烧烫伤
细菌感染
皮肤全切
损伤
愈合
superficial second degree burns and scalds
bacterial infection
total skin resection
wound
healing
作者简介
肖洒(1990-),男,硕士研究生,助理研究员,研究方向为实验动物模型及毒性病理学,E-mail:438799907@qq.com。;通信作者:张妙红(1982-),女,硕士研究生,副研究员,研究方向为毒性病理学,E-mail:zhangmiaohong@hnse.org。
