摘要
为明确氮硫互作对韭菜植株总辛辣程度和氮、硫素同化关键酶活性的影响,以韭菜品种‘京韭一号’为试材,采用水培模式处理,试验设定3个氮浓度(6、12和18 mmol/L),3个硫浓度(2、4和8 mmol/L),随机区组试验,在植株生长至第7、14、21和28天这4个不同生育周期节点时分别测定韭菜叶片的酶促丙酮酸(EPY)含量及硝酸还原酶(NR)、谷氨酰胺合成酶(GS)、ATP-硫酸化酶(ATPS)和氧乙酰丝氨酸(硫醇)裂解酶(OAS-TL)活性的动态变化。结果表明:EPY含量在植株生长至7、14与21 d时明显低于28 d;不同生育周期节点NR活性差异显著,氮硫素交互作用明显;GS活性响应程度剧烈并以植株长至28 d时水平最低;ATPS活性在植株长至7 d时水平最低且在中后期响应并不十分显著;OAS-TL活性呈现出平稳上升的趋势,但对氮硫素水平的敏感程度不及其他同化酶。韭菜发育过程中,氮素、硫素单一效应总体远低于元素间交互作用,氮硫素交互作用占据主导地位。韭菜植株硫素同化能力的提升与氮素水平密切相关,氮、硫素参与彼此的同化过程。综合考虑,建议水培韭菜营养液的氮素浓度为12 mmol/L,硫素浓度为2~4 mmol/L。该结论为水培韭菜氮、硫元素合理配施提供理论依据。
In order to clarify the effects of nitrogen and sulfur interaction on the total spiciness level of Chinese chive plants and the activities of key enzymes for nitrogen and sulfur assimilation,one Chinese chive variety‘Jingjiu No.1’was used as the test material and treated with hydroponic mode.Treatments of three different nitrogen concentrations(N1,N2,N3=6,12,18 mmol/L)and three different sulfur concentrations(S1,S2,S3=2,4,8 mmol/L)combination were set in a randomized block design.The dynamic changes of enzymatic pyruvate content(EPY)and activities of nitrate reductase(NR),glutamine synthetase(GS),ATP-sulfatase(ATPS)and oxyacetylserine(thiol)lyase(OAS-TL)in leaves of Chinese chives were determined at four different growth stages of 7 d,14 d,21 d and 28 d.The results showed that:The content of EPY at the first three periods of 7 d,14 d and 21 d was significantly lower than that at the 28 d period.There were significant differences in NR activity in different growth cycles,and the interaction between nitrogen and sulfur was obvious.The response of GS activity was intense and its lowest level occurred at 28 d.The activity of ATPS was the lowest at 7 d and the response was not very significant in the middle and late stages.The activity of OAS-TL showed a steady upward trend,but its sensitivity to nitrogen and sulfur was lower than other assimilation enzymes.In summary,during the development of Chinese chive,the single effect of either nitrogen or sulfur is generally much lower,and the interaction between nitrogen and sulfur is dominant.The improvement of sulfur assimilation ability of Chinese chive plants is closely related to nitrogen level,and nitrogen and sulfur participate in each other’s assimilation process.Considering comprehensively,the nitrogen concentration of the hydroponic Chinese chive nutrient solution is 12 mmol/L,and the sulfur concentration is 2 to 4 mmol/L is recommended.This study provides a theoretical basis for the rational application of nitrogen and sulfur elements in hydroponic Chinese chives.
作者
张烨达
佟静
王宝驹
刘宁
季延海
梁浩
王丽萍
武占会
ZHANG Yeda;TONG Jing;WANG Baoju;LIUNing;JI Yanhai;LIANG Hao;WANG Liping;WU Zhanhui(Beijing Vegetable Research Center/Key Laboratory of Urban Agriculture(North)of Ministry of Agriculture and Rural Affairs,Beijing Academy of Agriculture and Forestry Sciences,Beijing 100097,China;Collegeof Landscape and Ecological Engineering,Hebei University of Engineering,Handan 056038,China;State Assets and Base Management Division,Bejing Academy of Agricultural and Forestry Sciences,Beijing 100097,China)
出处
《中国农业大学学报》
CAS
CSCD
北大核心
2024年第8期95-108,共14页
Journal of China Agricultural University
基金
财政部和农业农村部:国家现代农业产业技术体系资助项目(CARS-24-B-02)
北京市农林科学院青年科研基金资助项目(QNJJ202248)
北京市农林科学院科技创新能力建设专项(KJCX20210402)
河北省现代农业产业技术体系露地蔬菜创新团队项目(HBCT2021200211)
河北省自然科学基金资助项目(C2021402014)
北京市农林科学院蔬菜研究中心改革与发展项目(KYCX202306)。
关键词
韭菜
氮
硫
辛辣程度
酶活性
互作效应
Chinese chives
nitrogen
sulfur
flavor intensity
enzyme activities
interaction effect
作者简介
第一作者:张烨达(ORCID:0009-0003-8142-8454),硕士研究生,E-mail:yedazhang2021@163.com.硕士研究生,中共预备党员,就读于河北工程大学园林与生态工程学院,主要研究方向为设施园艺与无土栽培。硕士期间,多次投身于新冠疫情防控志愿服务、学术志愿服务以及党支部主题活动,担任研究生“三助”岗位,并参加多项国内外学术报告会。荣获“疫情防控优秀志愿者”,现已连续三年获“研究生一等学业奖学金”,以第一作者在北大核心期刊发表2篇论文;通讯作者:佟静(ORCID:0009-0007-1963-2285),博士,助理研究员,主要从事蔬菜栽培生理与品质调控研究,E-mail:tongjing@nercv.org.博士,北京市农林科学院蔬菜研究所助理研究员,国家特色蔬菜产业技术体系土肥水管理岗位团队成员。研究方向为蔬菜水肥高效利用与品质调控。近年来主要从事非菜、加工辣椒、洋葱等特色蔬菜和莲藕、菱白、水芹等水生蔬菜的水肥高效利用与品质调控研究。曾参加十三五国家重点研发、十三五、十四五现代农业产业技术体系、林业公益项目以及北京园林绿化局、北京市农委等省部级项目10余项,主持院级项目2项。发表研究论文20余篇,其中SCI论文7篇,授权专利3项,参编行业标准1项;通信作者:武占会(ORCID:0000-0003-2753-0645),研究员,主要从事蔬菜设施栽培、无土栽培、蔬菜栽培生理等研究,E-mail:wuzhanhui@nercv.org,男,博士,北京市农林科学院研究员,国家特色蔬菜产业技术体系岗位科学家,中国园艺学会常务理事、中国农业工程学会设施园艺工程专委会委员、北京蔬菜学会常务理事。主要从事蔬菜设施栽培、无土栽培、蔬菜栽培生理等研究。主持多项科技部、农业农村部、北京市科委项目。获省级科技进步奖5项,发表科研论文110多篇,主编著作3部,发明专利4项,发布地方行业标准2项。
