摘要
为探索滩羊成纤维细胞生长因子5(fibroblast growth factor, FGF5)和辅酶Q9(coenzyme Q9,COQ9)基因的多态性及其与生长性状的关联性,运用分子标记辅助育种方法,为提高滩羊的生长性状提供理论依据。针对前期研究中筛选出的位点采用SNP分型技术对107只滩羊进行FGF5和COQ9基因多态性检测,并与初生重、二毛期(35日龄)、6月龄和12月龄体重进行关联分析。结果表明,通过与绵羊参考基因组(GCF_000298735.2_Oar_v4.0)比对,在滩羊FGF5基因上筛选出2个SNPs位点,分别为g.94540629T>G和g.94540642T>C,其中g.94540629T>G位点产生TT、GT和GG共3种基因型,g.94540642T>C位点产生TT、TC和CC共3种基因型;在COQ9基因上筛选出3个SNP位点,分别为g.94503480A>G、g.24807700A>G和g.24812228T>C,其中g.94503480A>G位点产生AA、AG、GG共3种基因型,g.24807700A>G位点产生AA、AG、GG共3种基因型,g.24812228T>C位点产生TT、TC和CC共3种基因型。基因多态性分析表明,5个SNPs位点属于高度多态,且在滩羊群体处于Hardy-Weinberg平衡状态(P>0.05);关联分析结果显示,FGF5基因g.94540642T>C位点C/T基因型12月体重显著大于其他基因型(P<0.05),COQ9基因g.24807700A>G位点GG基因型12月体重显著大于其他基因型(P<0.05),g.24812228T>C位点C/C基因型12月体重显著大于其他基因型(P<0.05),由此可知,3个SNP位点对滩羊12月龄体重有显著影响;COQ9基因g.24812228T>C和g.24807700A>G位点在滩羊群体处于强连锁不平衡,FGF5基因g.94540629T>G和g.94540642T>C位点在滩羊群体处于强连锁不平衡,COQ9基因g.94503480A>G、g.24807700A>G和g.24812228T>C位点和基因型组合A/GA/AT/T与A/GA/GT/C在出生重、6月体重和12月体重显著高于其他组合基因型(P<0.05)。FGF5和COQ9基因多态性与滩羊生长性状显著相关,FGF5和COQ9基因可作为滩羊生长性状选育的候选基因,可作为滩羊体重性状的分子标记,通过常规育种结合分子标记辅助选择来提高滩羊的选育效率。
To explore the polymorphisms of fibroblast growth factor 5(FGF5)and coenzyme Q9(COQ9)genes and their associations with growth traits in Tan sheep fibroblast growth factor(FGF5)and coenzyme Q9(COQ9),so as to provide a theoretical basis for the use of molecular marker-assisted breeding methods to improve the growth traits of Tan sheep,the polymorphisms of FGF 5 and COQ 9 genes were detected in 107 Tan sheep using SNP typing technology for the loci screened in the previous study,and the body weight of primary birth,35 days old,6 months old and 12 months old was analyzed.The results showed that two SNPs were screened on the FGF 5 gene of Tan sheep by alignment with the reference genome of sheep(GCF_000298735.2_Oar_v4.0),which were g.94540629 T>G and g.94540642 T>C,where g.Three genotypes,TT,GT and GG,were produced g.94540629 T>G,and three genotypes of TT,TC and CC were generated at the g.94540642 T>C loci.Three SNPs were screened in the COQ 9 gene,which were g.94503480 A>G,g.24807700 A>G and g.24812228 T>C,where three genotypes of AA,AG and GG were produced at the g.94503480 A>G loci,three genotypes of AA,AG and GG3 were generated at the g.24807700 A>G loci,and three genotypes of TT,TC and CC were generated at the g.24812228 T>C loci.Gene polymorphism analysis showed that the five SNPs loci were highly polymorphic and in the Hardy-Weinberg equilibrium state in the Tan sheep population(P>0.05).The results of association analysis showed that the weight of the g.94540642 T>C locus of FGF 5 gene C/T genotype at 12 months was significantly greater than that of other genotypes(P<0.05),the body weight of GG genotype at the g.24807700 A>G locus of COQ 9 gene was significantly greater than that of other genotypes at 12 months(P<0.05),and the body weight of g.24812228 T>C loci C/C genotype at 12 months was significantly greater than that of other genotypes(P<0.05),it was concluded that the three SNP loci had a significant effect on the body weight of Tan sheep at 12 months,the COQ 9 gene g.24812228 T>C and g.24807700 A>G loci were in strong linkage imbalance in the Tan sheep population,the FGF 5 gene g.94540629 T>G and g.94540642 T>C loci were in strong linkage imbalance in the Tan sheep population,and the COQ9 gene g.94503480 A>G,g.24807700 A>G and g.24812228 T>C locus genotypes combination A/GA/AT/T and A/GA/GT/C were significantly higher than those of other combination genotypes at birth weight,6 months weight and 12 months weight(P<0.05).FGF 5 and COQ 9 gene polymorphisms were significantly correlated with the growth traits of Tan sheep,and the FGF 5 and COQ 9 genes could be used as candidate genes for the breeding of growth traits and molecular markers of weight traits in Tan sheep,and the breeding efficiency of Tan sheep could be improved by conventional breeding combined with molecular marker-assisted selection.
作者
马丽娜
田进阳
王锦
赵正伟
马青
MA Li na;TIAN Jinyang;WANG Jin;ZHAO Zhengwei;MA Qing(Institute of Animal Sciences,Ningxia Academy of Agricultural and Forestry Sciences,Yinchuan 750002,China;Tan Sheep Breeding Farm in Ningxia Hui Autonomous Region,Wuzhong 751102,Ningxia,China)
出处
《浙江农业学报》
CSCD
北大核心
2024年第5期1015-1023,共9页
Acta Agriculturae Zhejiangensis
基金
宁夏回族自治区重点研发计划项目(2023BCF01007)。
作者简介
马丽娜(1985-),女,宁夏石嘴山人,硕士,副研究员,研究方向为动物遗传育种。E-mail:malina_2007nian@163.com;通信作者:马青,E-mail:maqing1973@126.com。
