摘要
目的 对布鲁氏菌T4SS 15个效应蛋白进行生物信息学分析。方法 从NCBI数据库中获取布鲁氏菌T4SS的15个效应蛋白的氨基酸序列,利用生物信息学软件分析15个效应蛋白的理化性质及同源建模分析跨膜区、信号肽、亲水性、保守结构域、B细胞抗原表位、T细胞抗原表位、抗原性、翻译后修饰位点及效应蛋白间的相互作用等。结果 15个布鲁氏菌T4SS效应蛋白主要为无信号肽和跨膜螺旋结构的亲水性蛋白,其中BspB有跨膜螺旋结构和信号肽、RicA、BspA、BPE005和BPE275疏水性蛋白;除VceC、BspB、BspC、BspE和BPE123外其余11个效应蛋白均具有1个或多个稳定结构域;BPE043有优势B细胞抗原表位且CD4 T细胞抗原表位数量较高;修饰化位点预测显示:15个效应蛋白中有12个同时具有糖基化、甲基化、磷酸化、乙酰化修饰位点;对效应蛋白互作预测分析显示BPE043可同时与VceC、BspE、BspF蛋白发生互作,其余11种效应蛋白间发生互作的概率较低。结论 BPE043蛋白抗原性较强,可作为布鲁氏菌潜在的疫苗候选基因,为布鲁氏菌T4SS效应蛋白生物学功能研究提供科学依据。
Objective Using bioinformatics softwaretoanalysis 15 effector proteins of Brucella T4SS.Methods The amino acid sequences of Brucella T4SS effector proteins were obtained from the NCBI database,and analyze the physicochemical properties,homologous modeling,transmembrane domain,signal peptide,hydrophilicity,conserved domain,B cell antigen epitopes,T cell antigen epitopes,antigenicity,post-translational modification sitesand proteinprotein interactions.using bioinformatics software to analyze.Results Most of the 15 T4SS effector proteins are hydrophilic proteins without signal peptides and transmembrane domains,BspB has transmembrane helix structure and signal peptide,except for RicA,BspA,BPE005 and BPE275 are hydrophilic proteins.Except for VceC,BspB,BspC,BspE and BPE123,the other 11 effector proteins have one or more stabilizing structural domains.BPE043 has a superior B cell antigen epitope and a higher number of CD4 T cell antigen epitopes.Modification site prediction showed that 12 of the 15 effector proteins have glycosylation,methylation,phosphorylation,and acetylation modification sites.Predictive analysis of the interaction between effector proteins showed that BPE043 can interact with VceC,BspE,and BspF,but has less interaction with the other 11 effector proteins..Conclusion The BPE043 protein has strong antigenicity and can be used as a potential candidate gene for Brucellosis vaccine,providing scientific basis for the study of the biological function of Brucella T4SS effector proteins.
作者
谢珊珊
邓肖玉
彭泽宇
孙天浩
王震
陈创夫
XIE Shanshan;DENG Xiaoyu;PENG Zeyu;SUN Tianhao;WANG Zhen;CHEN Chuangfu(College of Animal Science and Technology,Shihezi University,Shihezi 832000,Xinjiang,China;Key Laboratory of Animal Disease Prevention and Control Corps)
出处
《中国病原生物学杂志》
CSCD
北大核心
2024年第7期800-805,共6页
Journal of Pathogen Biology
基金
国家自然科学基金项目(No.32002245,U1803236)
兵团重大科技项目(No.2017AA003)。
作者简介
通讯作者:陈创夫,E-mail:ccf-xb@163.com;通讯作者:王震,E-mail:wzhen2018@shzu.edu.cn;谢珊珊(1997-),女,河南商丘人,硕士研究生,研究方向:预防兽医学。E-mail:1752688152@qq.com。
