摘要
微流控芯片是集成化、高通量、低成本、便携式的生物分析检测工具,近年来在新型冠状病毒(SARS-CoV-2)检测中备受关注。本文综述了微流控芯片及其系统在SARS-CoV-2检测中的最新进展,介绍并对比了微流控芯片的制备材料和成型工艺,着重阐述了基于微流控芯片的核酸分子检测、血清学检测和即时检测(POCT)技术的应用研究进展,为检测方法的创新设计和芯片制备提供参考。将现有生物检测技术与微流控设备有效结合,可以实现SARS-CoV-2的高效、灵敏和准确检测,基于微流控芯片的病毒检测方法呈现出多样化、集成化的特点,是传统病毒检测方法的有效替代方案。本文探讨了各种检测技术的适用性和现有挑战,将帮助学者开展更为广泛深入的生物医学分析检测研究,深化认识先进检测技术与微流控技术的发展前景。
Regarded as an integrated,high-through put and low-cost biological analysis and detection tool,microfluidic chip has attracted much attention in the detection of SARS-CoV-2 in recent years.Firstly,the latest progress of microfluidic chip and its systems in SARS-CoV-2 testing are reviewed,and we also describe and compare the materials and preparation process of microfluidic chip.Then,we focus on detection technologies based on microfluidic chip such as nucleic acid molecular tests,serological tests and point of care tests(POCT),which provides reference value for innovative design of detection method and chip preparation.The combination of biological detection technology and microfluidics equipment could effectively realize efficient,sensitive and accurate detection of SARS-CoV-2.Virus testing methods based on microfluidic chip would be an effective alternative to the traditional virus testing method,which presents the characteristics of diversification and integration.Lastly,the applicability and current challenges of various detection technologies are also discussed,which would help scholars to carry out more extensive and in-depth biomedical analysis and detection research and deepen their understanding of the development prospects of advanced detection technologies and microfluidic technologies.
作者
陈润华
戴永祺
张建业
李方德
鲁艳军
CHEN Runhua;DAI Yongqi;ZHANG Jianye;LI Fangde;LU Yanjun(College of Mechatronics and Control Engineering,Shenzhen University,Shenzhen 518060,Guangdong,China;Guangdong Provincial Key Laboratory of Molecular Target and Clinical Pharmacology,School of Pharmaceutical Sciences,Guangzhou Medical University,Guangzhou 511436,China)
出处
《材料导报》
EI
CAS
CSCD
北大核心
2024年第12期32-43,共12页
Materials Reports
基金
国家自然科学基金(51805334)
广东省科技计划项目(2022A0505050080)。
作者简介
陈润华,现为深圳大学机电与控制工程学院硕士研究生,2021年6月在华南农业大学机械设计制造及其自动化专业取得学士学位。目前主要研究领域为微流控芯片设计与制造、微结构精密加工技术等;通信作者:鲁艳军,深圳大学机电与控制工程学院长聘副教授、硕士研究生导师。2015年6月在华南理工大学机械制造及其自动化专业取得博士学位。主要研究方向为精密/超精密加工工艺与装备、微纳结构加工、微流控芯片制造等。主持和完成国家自然科学基金、中国博士后科学基金、广东省科技计划项目、深圳市技术攻关重点项目等20余项,在International Journal of Machine Tools and Manufacture、《机械工程学报》等期刊发表学术论文20余篇,获授权发明专利20项。luyanjun@szu.edu.cn,luyanjun_szu@163.com。
