摘要
目的探讨circ_0013958对子宫内膜癌细胞RL-95-2增殖、凋亡和迁移影响及可能机制。方法收集51例子宫内膜癌组织和癌旁组织,即时聚合酶链锁反应(qRT-PCR)法检测组织中circ_0013958和miR-545表达。体外培养RL-95-2细胞,分别转染sh-circ_0013958、miR-545模拟物、si-circ_0013958与miR-545抑制剂后,CCK-8、流式细胞术、Transwell分别检测细胞增殖、凋亡和迁移。凋亡蛋白水平使用Western blot。Circ_0013958和miR-545的靶向关系使用双荧光素酶报告实验验证。结果子宫内膜癌组织中相较于癌旁组织高表达circ_0013958(P<0.05),而低表达miR-545(P<0.05);敲减circ_0013958或上调miR-545后,RL-95-2细胞增殖及迁移能力抑制而细胞凋亡升高(P<0.05);circ_0013958靶向负调控miR-545,下调miR-545逆转了敲减circ_0013958介导的抗增殖,抗迁移以及促凋亡的作用。结论circ_0013958通过靶向下调miR-545促进子宫内膜癌RL-95-2细胞增殖和迁移,并阻碍细胞凋亡。
Objective To explore the effects of circ_0013958 on the proliferation,apoptosis and migration of endometrial cancer cell RL-95-2 and its possible mechanism.Methods Endometrial cancer tissues and paracancerous tissues of 51 cases were collected,and the expression levels of circ_0013958 and miR-545 in the tissues were detected by quantitative real time polymerase chain reaction(qRT-PCR).RL-95-2 cells were cultured in vitro and transfected with sh-circ_0013958,miR-545 mimics,sicirc_0013958 and miR-545 inhibitor respectively,and then cell proliferation,apoptosis,and migration were detected by CCK-8,flow cytometry,and Transwell assays,respectively.The levels of apoptosis-related proteins were detected by Western blot.The targeting relationship between circ_0013958 and miR-545 was verified by the dual luciferase reporter assay.Results Compared with paracancerous tissues,the expression level of circ_0013958 in endometrial cancer tissues was higher(P<0.05),and the expression level of miR-545 was lower(P<0.05).After knocking down circ_0013958 or up-regulating miR-545,the proliferation and migration ability of RL-95-2 cells was inhibited,while cell apoptosis was increased(P<0.05).circ_0013958 targeted negative regulation of miR-545.Down-regulation of miR-545 reversed the anti-proliferation,anti-migration and pro-apoptosis effects mediated by knocking down circ_0013958.Conclusion circ_0013958 may promote the proliferation and migration of endometrial cancer RL-95-2 cells and hinder cell apoptosis by targeting down-regulation of miR-545.
作者
张仕田
何翔
杨帆
赵红利
龚钿
ZHANG Shitian;HE Xiang;YANG Fan;ZHAO Hongli;GONG Dian(Department of Obstetrics and Gynecology,Meishan People's Hospital of Sichuan Province,Meishan 620010,China)
出处
《长春中医药大学学报》
2024年第7期746-750,共5页
Journal of Changchun University of Chinese Medicine
基金
四川省科技计划项目(2021YJ0137)。
作者简介
张仕田(1979-),男,硕士,副主任医师,主要从事妇科肿瘤方面研究;通信作者:杨帆,女,副主任医师,电子信箱-sharry48@163.com。
