摘要
【目的】滨海湿地生态系统位于淡水与海水交互地带,含有高浓度Fe^(2+)的地下水渗透到沉积物表层形成的湿地径流和周期性潮汐淹水形成的含氧-缺氧界面有利于铁氧化细菌介导的Fe^(2+)的生物氧化过程发生。然而,目前缺乏对滨海湿地生态系统中铁氧化细菌类群的全面评估。【方法】以上海崇明西沙湿地公园及浙江舟山市朱家尖岛东沙沙滩两地共5处滨海湿地沉积物为研究对象,分析沉积物的氧气穿透深度等环境参数,并基于16S rRNA基因扩增子测序技术,全面解析不同滨海湿地生态系统中细菌与铁氧化细菌的群落组成与分布特征。【结果】与崇明西沙湿地相比,朱家尖岛东沙沙滩有更深的氧气穿透深度,达到10 mm以上。非度量多尺度分析(non-metric multidimensional scaling,NMDS)统计结果表明,细菌群落结构主要受到区域位置不同导致的环境条件差异的影响,而铁氧化细菌的群落结构则受到采样的区域位置和沉积物氧气穿透深度的共同影响。崇明西沙湿地和朱家尖岛东沙沙滩的优势细菌为蓝细菌门(Cyanobacteria)、γ-变形菌纲(Gammaproteobacteria)、拟杆菌门(Bacteroidetes)、α-变形菌纲(Alphaproteobacteria)和放线菌门(Actinobacteria);优势铁氧化细菌为嘉利翁氏菌属(Gallionella)、红细菌属(Rhodobacter)、Lepthothrix和Sideroxydans。【结论】通过对崇明西沙湿地和朱家尖岛东沙沙滩沉积物中栖息的铁氧化菌的调查发现,铁氧化细菌的群落组成与湿地沉积物类型导致的氧气穿透深度差异具有密切联系。
[Objective]Coastal wetland ecosystems,situated at the interface of freshwater and seawater,are characterized by the seepage of groundwater with high Fe^(2+)concentrations into the surface layers of sediments,which forms wetland runoff.This runoff,combined with periodic tidal flooding,creates an oxic-anoxic interface conducive to the bio-oxidation of Fe^(2+)by Fe-oxidizing bacteria.However,there is a lack of comprehensive assessment of Fe-oxidizing bacterial communities in coastal wetland ecosystems.[Methods]We measured the basic environmental parameters such as the oxygen penetration depth in the sediments of five coastal wetland sites in Xisha Wetland Park in Chongming,Shanghai and Dongsha Beach in Zhujiajian Island in Zhoushan,Zhejiang.The community composition and distribution of bacteria and Fe-oxidizing bacteria were comprehensively deciphered by 16S rRNA gene amplicon sequencing.[Results]Dongsha Beach in Zhujiajian Island exhibited deeper oxygen penetration(reaching more than 10 mm)than Xisha Wetland in Chongming.The non-metric multidimensional scaling(NMDS)results indicated that the bacterial community structure was primarily influenced by environmental conditions that varied with geographical location,while the community structure of Fe-oxidizing bacteria was influenced by both the geographical location of the sampling sites and the oxygen penetration depth of the sediments.The dominant bacteria in Xisha Wetland and Dongsha Beach were Cyanobacteria,Gammaproteobacteria,Bacteroidetes,Alphaproteobacteria,and Actinobacteria.The dominant genera of Fe-oxidizing bacteria were Gallionella,Rhodobacter,Lepthothrix,and Sideroxydans.[Conclusion]We studied the Fe-oxidizing bacteria in the sediments of Xisha Wetland in Chongming and Dongsha Beach in Zhujiajian Island and discovered that the composition of Fe-oxidizing bacterial communities was closely linked to the oxygen penetration depth variations caused by different types of wetland sediments.
作者
祁文龙
宿蕾
马忠霖
王鹏
李江涛
QI Wenlong;SU Lei;MA Zhonglin;WANG Peng;LI Jiangtao(State Key Laboratory of Marine Geology,Tongji University,Shanghai 200092,China)
出处
《微生物学报》
CAS
CSCD
北大核心
2024年第6期1704-1720,共17页
Acta Microbiologica Sinica
基金
国家自然科学基金(42072333)。
关键词
崇明西沙湿地
朱家尖岛东沙沙滩
滨海湿地
氧气穿透深度
铁氧化细菌
Xisha Wetland in Chongming
Dongsha Beach in Zhujiajian Island
coastal wetlands
oxygen penetration depth
Fe-oxidizing bacteria
作者简介
Corresponding author:李江涛.E-mail:jtli@tongji.edu.cn。
