摘要
[目的]试验研究L—半胱氨酸、精氨酸对牛精液冷冻效果。[方法]基础液中分别添加不同浓度的L—半胱氨酸、精氨酸进行试验,再用L_(9)(3^(4))正交表优化最佳配比,获得不同的稀释液配方,然后再进行对比试验,优选出使用效果最好的牛精液冷冻稀释液。[结果]牛精液冷冻稀释液添加0.125%、0.25%、0.375%的半胱氨酸;牛精液冷冻稀释液添加0.5%、0.75%精氨酸,牛冷冻精液解冻活力均得到提高。[结论]结果表明,半胱氨酸、精氨酸可用于牛精液冷冻保存,能改善牛精液冷冻保存效果,对提高牛冷冻精液质量效果明显。
This study was to investigate the effect of L-cysteine and arginine on bovine semen freezing.Different concentrations of L-cysteine and arginine were added to the base solution for testing,and then the optimal ratio was optimized by L_(9)(3^(4))orthogonal table to obtain the different diluent formulas,and then the comparative test was carried out to select the best frozen dilution of bovine semen.The thawing vitality of bovine frozen semen was improved by adding with 0.125%,0.25%and 0.375%cysteine,and{0.5%}and 0.75%arginine.The results showed that cysteine and arginine could be used for the cryopreservation of bovine semen,which could improve the cryopreservation effect of bovine semen,and had obvious effects on improving the quality of bovine frozen semen.
作者
李雯
杨强
权约海
李贵有
李春平
李鸿
朱启耀
黄必志
赵家才
LI Wen;YANG Qiang;QUAN Yue-hai;LI Gui-you;LI Chun-ping;LI Hong;ZHU Qi-yao;HUANG Bi-zhi;ZHAO Jia-cai(Yunnan Vocational College of Agriculture,Kunming,Yunnan 650212;Academe of Grassland and Animal Science,Kunming,Yunnan 650212)
出处
《中国牛业科学》
2024年第3期30-32,43,共4页
China Cattle Science
基金
云南农业职业技术学院科研项目,编号:Ynavc202214。
作者简介
李雯(1968-),女云南省昆明人,高级畜牧师,主要从事家畜冷冻精液技术的研究、冷冻精液生产和质量管理工作;通讯作者:黄必志(1964-),研究员.博士生导师,主要从事动物遗传育种、饲草资源开发与评价,动物营养与饲料研究;通讯作者:赵家才(1965-),男云南禄劝人,高级畜牧师主要从事家畜冷冻精液技术研究,牛品种改良工作。
