摘要
通过在维生素C二步种液培养基中添加半胱氨酸或(和)氯化钙的方法,研究了这2种物质对二步菌种生长和产2-酮基-L-古龙酸(2-KLG)的影响。结果表明,在二步种子培养基中加入适量的半胱氨酸和(或)氯化钙能够促进普通生酮基古龙酸菌生长,提高菌体密度,协调混菌比例,提高种子质量,并提高后续发酵2-酮基-L-古龙酸生成速率,缩短发酵周期。在种子培养基中同时加入0.3 g/L半胱氨酸和0.6 g/L氯化钙培养效果最显著:种液中普通生酮基古龙酸菌浓度提高30.77%,发酵周期缩短8.33%,2-酮基-L-古龙酸生成速率提高9.31%。
The effects of L-cysteine or(and)calcium chloride on the growth and production of 2-keto-L-gulonic acid(2-KLG)in two-step culture medium of vitamin C were studied.The results showed that adding appropriate amount of L-cysteine and(or)calcium chloride to the two-step culture medium could promote the growth of Ketogulonigenium vulgare,increase the cell density,coordinate the mixed bacteria proportion and improve the quality of the mixed strains,the production rate of 2-keto-L-gulonic acid was increased and the fermentation period was shortened.The effect of adding 0.3 g/L cysteine and 0.6 g/L calcium chloride in the seed liquor culture medium was the most remarkable.The concentration of Ketogulonigenium vulgare in the seed liquor increased by 30.77%,and the fermentation period shortened by 8.33%,the formation rate of 2-keto-L-gulonic acid increased by 9.31%.
作者
胡静
刘丽华
朱娜
苏宏儒
王婧
米帅东
Hu Jing;Liu Lihua;Zhu Na;Su Hongru;Wang Jing;Mi Shuaidong(CSPC Weisheng Pharmaceutical(Shijiazhuang)Corporation Ltd.,Shijiazhuang 050000,China;Shijiazhuang Food and Drug Inspection Center Shijiazhuang 050000,China;Shijiazhuang Vitamin C Technology Innovation Center,Shijiazhuang 050000,China;Hebei Province Nutrition Fortifier Technology Innovation Center,Shijiazhuang 050000 China)
出处
《煤炭与化工》
CAS
2024年第5期144-147,共4页
Coal and Chemical Industry
作者简介
胡静(1978-),女,河北石家庄人,工程师。
