摘要
为探讨不同水平的枯草芽孢杆菌(Bacillus subtilis)对南美白对虾(Litopenaeus vannamei)机体非特异性免疫力的影响,该试验选择600尾体重约为0.45g健康的南美白对虾,按照单因素试验设计,分成4个组,每组3个重复,每个重复50尾南美白对虾。各组分别投喂含有0%(对照组)、0.5%、1.0%和1.5%的枯草芽孢杆菌的基础饲料。投喂8周后,采集血液,检测血清非特异性免疫指标。结果显示,与对照组比较,试验2组的南美白对虾血清中溶菌酶、碱性磷酸酶、酸性磷酸酶、酚氧化物酶活性显著提高(P<0.05),试验2组和试验3组南美白对虾血清中过氧化氢酶和超氧化物歧化酶活性显著提高(P<0.05)。研究表明,在南美白对虾养殖生产中添加一定剂量的枯草芽孢杆菌可以提高机体的非特异性免疫力,且适宜添加量为1.0%。
In order to explore the effects of different levels of Bacillus subtilis on the nonspecific immunity of the organism of Litopenaeus vannamei,600 healthy Litopenaeus vannamei weighing about 0.45 g were selected for this experiment,which were divided into four groups of three replicates each according to a one-way experimental design,with each replicate of each group was divided into four groups with three replicates,each with 50 shrimps.Each group was fed a basal diet containing 0%(control),0.5%,1.0%and 1.5%Bacillus subtilis,respectively.After 8 weeks of feeding,blood was collected and tested for serum non-specific immunity indexes.The results showed that lysozyme,alkaline phosphatase,acid phosphatase,and phenoloxidase activities were significantly higher(P<0.05)in the serum of Litopenaeus vannamei in test group 2 compared with the control group,and catalase and superoxide dismutase activities were significantly higher(P<0.05)in the serum of Litopenaeus vannamei in both test group 2 and test group 3.The study showed that the addition of a certain dose of Bacillus subtilis in the production of Litopenaeus vannamei culture can improve the non-specific immunity of the organism,and the appropriate amount is 1.0%.
作者
魏春丽
WEI Chunli(Helan County Agriculture and Rural Affairs Bureau,Yinchuan 750200,Ningxia China)
出处
《黑龙江水产》
2024年第3期294-296,共3页
Northern Chinese Fisheries
作者简介
魏春丽(1983.2-)女、汉族,辽宁人。主要从事水产养殖技术工作。E-mail:470516089@qq.com。
