摘要
为明确国审小麦新品种信麦163的分子遗传基础,利用小麦55K SNP育种芯片对信麦163及其母本信阳234和父本丰抗38进行分析。结果表明,信阳234和丰抗38对信麦163的遗传贡献率分别为49.60%和50.40%。在基因组和染色体水平上,双亲对信麦163的遗传贡献率差异较大:母本信阳234对信麦163 A、B、D三个基因组的贡献率分别为49.34%、52.52%和45.61%,贡献率超过50%的染色体有4A、5A、7A、4B、5B、6B、7B、1D、5D、6D和7D,其中在7A、7B、7D上遗传贡献率超过60%;父本丰抗38对信麦163 A、B、D三个基因组的贡献率分别为50.66%、47.48%和54.39%,贡献率超过50%的染色体有1A、2A、3A、6A、1B、2B、3B、2D、3D和4D,其中在4D染色体上遗传贡献率超过80%。在3A、4D、6B等染色体上的遗传形式主要表现为亲本遗传信息以染色体大片段形式传递到子代。SNP(单核苷酸多态性)基因型图谱、SNP位点分析与遗传贡献率分析结果具有较好的一致性。本研究结果展示了杂交育种对后代基因组造成的影响,可为信麦163在遗传改良和生产中的应用提供科学依据。
In order to clarify the molecular genetic basis of the new wheat variety Xinmai 163,it and its female parent Xinyang 234 and male parent Fengkang 38 were analyzed using the wheat 55K SNP array.The results showed that the genetic contribution ratios of Xinyang 234 and Fengkang 38 to Xinmai 163 were 49.60%and 50.40%,respectively.The genetic contribution from parents to Xinmai 163 varied greatly at the genome and chromosome levels.The female parent Xinyang 234 contributed 49.34%、52.52%and 45.61%to genomes A,B and D of Xinmai 163,respectively.Chromosomes 4A,5A,7A,4B,5B,6B,7B,1D,5D,6D and 7D contributed more than 50%,among which,chromosomes 7A,7B and 7D contributed more than 60%.The male parent Fengkang 38 contributed 50.66%,47.48%and 54.39%to genomes A,B and D of Xin-mai 163,respectively.Chromosomes 1A,2A,3A,6A,1B,2B,3B,2D,3D and 4D contributed more than 50%,among which,Chromosome 4D contributed more than 80%.The genetic information in chromosomes 3A,4D and 6B of parents was transmitted to offspring mainly in forms of large segments of chromosomes.The results of single nucleotide polymorphism(SNP)genotype map,SNP locus analysis and genetic contribution analysis showed good consistency.This study showed the effect of cross breeding on genome of offspring,which could provide a scientific basis for the application of Xinmai 163 in genetic improvement and production.
作者
陈真真
李杰
王轲
陈金平
申冠宇
谢旭东
石守设
杨军
周国勤
Chen Zhenzhen;Li Jie;Wang Ke;Chen Jinping;Shen Guanyu;Xie Xudong;Shi Shoushe;Yang Jun;Zhou Guoqin(Xinyang Academy of Agricultural Sciences,Xinyang 464000,China;Xinyang Hongrun Family Farm Co.,Ltd.,Xinyang 464000,China)
出处
《山东农业科学》
北大核心
2024年第5期42-48,共7页
Shandong Agricultural Sciences
基金
河南省“揭榜挂帅”技术攻关专项“豫南酿酒专用弱筋小麦优质高效产业化集成技术研究与规模化应用”(21110110800)
国家小麦产业技术体系专项“小麦全产业链绿色优质高效生产技术模式研究”(CARS-03-01A)
河南省小麦产业技术体系项目。
作者简介
陈真真(1990-),女,河南驻马店人,硕士,助理研究员,主要从事小麦遗传育种研究。E-mail:396939159@qq.com;通信作者:周国勤(1974-),女,河南信阳人,本科,副研究员,主要从事小麦遗传育种与栽培研究。E-mail:zhouguoqin74@126.com。
