摘要
目的调查医院和宾馆公共电梯按钮金黄色葡萄球菌的污染状况,并观察金黄色葡萄球菌对常用消毒剂的抗性。方法采用Baird-Parker筛选培养基分离金黄色葡萄球菌,通过聚合酶链式反应对消毒剂抗性基因qacA/B、smr进行扩增,用最小抑菌浓度(MIC)和最小杀菌浓度(MBC)评价金黄色葡萄球菌对常用消毒剂的抗性。结果从83份公共电梯按钮样本中共检出金黄色葡萄球菌阳性样本21份,检出率为25.3%。医院和宾馆电梯按钮金黄色葡萄球菌的检出率分别为10.0%和39.5%,差异有统计学意义(P=0.002)。分离出的21株金黄色葡萄球菌均未检测出基因qacA/B、smr,但一些菌株对季铵盐消毒剂的抗力要高于标准菌株。结论宾馆电梯按钮金黄色葡萄球菌的污染状况比医院电梯按钮更加严重,发现部分金黄色葡萄球菌对季铵盐消毒剂产生了抗性。
Objective To investigate the contamination status of Staphylococcus aureus on public elevator buttons of hospitals and hotels,and to observe the resistance of Staphylococcus aureus to commonly used disinfectants.Methods Baird-Parker screening medium was used to isolate Staphylococcus aureus,and the disinfectant resistance genes qacA/B,smr were amplified by polymerase chain reaction.The resistance of Staphylococcus aureus to commonly used disinfectants was tested using minimal inhibitory concentration(MIC)and minimum bactericidal concentrations(MBC).Results A total of 21 strains of Staphylococcus aureus were detected on 83 public elevator buttons,with a detection rate of 25.3%.The detection rates of Staphylococcus aureus on the elevator buttons of hospitals and hotels were 10.0%and 39.5%,respectively,with a statistically significant difference(P=0.002).The genes qacA/B,smr were not detected in the 21 strains of Staphylococcus aureus isolated,but some strains showed higher resistance to quaternary ammonium disinfectants than the standard strains.Conclusion The contamination of hotel elevator buttons by Staphylococcus aureus is more severe than that of hospital elevator buttons,and it has been found that some Staphylococcus aureus had developed resistance to quaternary ammonium salt disinfectants.
作者
叶珊珊
吴晓松
彭世富
王晓蕾
茅一萍
范晶晶
黄立业
王路梅
倪婷婷
朱陈雪
徐燕
YE Shan-shan;WU Xiao-song;PENG Shi-fu;WANG Xiao-lei;MAO Yi-ping;FAN Jing-jing;HUANG Li-ye;WANG Lu-mei;NI Ting-ting;ZHU Chen-xue;XU Yan(School of Public Health Nanjing Medical University,Nanjing Jiangsu 211166;Jiangsu Provincial Center for Disease Control and Prevention;Jiangsu Provincial Medical Key Laboratory of Pathogenic Microbiology in Emerging Major Infectious Diseases;National Health Committee Key Laboratory of Enteric Pathogenic Microbiology;The Affiliated Hospital of Xuzhou Medical University;Xuzhou Municipal Center for Disease Control and Prevention,China)
出处
《中国消毒学杂志》
CAS
2024年第4期283-286,共4页
Chinese Journal of Disinfection
基金
江苏省医学科研重点基金资助项目(ZDA2020015,LGY2019074,K2019008)。
关键词
金黄色葡萄球菌
消毒剂
抗性
Staphylococcus aureus
disinfectant
resistance
作者简介
叶珊珊(1998-),女,安徽定远人,硕士研究生在读,从事消毒与医院感染控制研究;通信作者:徐燕,E-mail:cdcxy@vip.sina.com。
