摘要
为了充分利用丹皮活性成分资源,本文采用40%、70%和95%的乙醇对丹皮中活性成分进行超声提取,分别得到丹皮40%、70%和95%乙醇提取物(MC 40、MC 70和MC 95),比较了提取物中的总酚和总黄酮含量,以及体外抗氧化能力和抗糖尿病活性,最后采用斑马鱼模型评价了提取物的体内降血糖和外周运动神经保护活性。结果表明:MC 70具有最高的总酚(59.17 mg GAE/g,没食子酸当量)和总黄酮(82.59 mg QUE/g,槲皮素当量)含量,同时具有最高的抗氧化活性,其清除DPPH自由基和ABTS自由基的IC 50值分别为34.20μg/mL和16.30μg/mL,MC 40抗氧化活性最差。MC 95的糖基化抑制率最高,达95.92%,其次为MC 70(81.44%)。高血糖斑马鱼模型实验结果表明,MC 70干预后,与模型组相比血糖值降低了52%,外周运动神经损伤程度降低了56%。因此,70%的乙醇可作为提取丹皮中抗氧化和降血糖成分的适宜提取溶剂,提取物具有较好的抗氧化和抗糖尿病潜力,为丹皮后续的高值化利用提供理论基础。
This study aimed to fully utilize the resources of Moutan Cortex,40%,70%and 95%ethanol solutions were used to extract the active components of Moutan Cortex by ultrasound,and 40%,70%and 95%ethanol extracts(MC 40,MC 70 and MC 95)were obtained,respectively.The contents of total phenols and total flavonoids,as well as the antioxidant capacity and anti-diabetic activity in vitro of the three extracts were compared.Finally,the zebrafish model in vivo was used to evaluate the hypoglycemic and peripheral motor neuroprotective activities of the extracts.The results show that MC 70 had the highest content of total phenol(59.17 mg GAE/g,gallic acid equivalent)and total flavone(82.59 mg QUE/g,quercetin equivalent).Meanwhile,MC 70 exhibited the strongest antioxidant activity,with IC 50 values of 34.20μg/mL and 16.30μg/m for DPPH and ABTS free radical scavenging capacities,respectively,and MC 40 showed the weakest antioxidant activity.In addition,MC 95 exhibited the highest inhibition rate of glycosylation(95.92%),followed by MC 70(81.44%).The experimental results of the hyperglycemic zebrafish model showed that MC 70 treatment resulted in a 52%decrease in blood glucose levels and a 56%reduction in peripheral motor nerve injury,compared with the model group.The results suggest that 70%ethanol was the suitable solvent for extracting antioxidant and hypoglycemic components from Moutan Cortex,and the extract has good antioxidant and anti-diabetic potential,providing a theoretical basis for the subsequent high-value utilization of the peel.
作者
张露
程鑫鹏
邓梅
陈明舜
周祺溟
彭春彦
谢作桦
涂宗财
ZHANG Lu;CHENG Xin-peng;DENG Mei;CHEN Ming-shun;ZHOU Qi-min;PENG Chun-yan;XIE Zuo-hua;TU Zong-cai(National R&D Center for Freshwater Fish Processing,College of Life Science,Jiangxi Normal University;College of Health,Jiangxi Normal University,Nanchang 330022,China;Jiangxi Deshang Pharmaceutical Co.,Ltd.,Zhangshu 331200,China)
出处
《天然产物研究与开发》
CAS
CSCD
北大核心
2024年第5期762-770,共9页
Natural Product Research and Development
基金
国家自然科学基金(32301993)
江西省自然科学基金(20232BAB205072)。
关键词
丹皮
多酚类化合物
抗氧化
斑马鱼
降血糖
Moutan Cortex
polyphenols
antioxidant
zebrafish
anti-diabetes
作者简介
通信作者:陈明舜,E-mail:chenshun1221@163.com。
