摘要
目的:探讨新型冠状病毒感染患者感染入院时及SARS-CoV-2核酸转阴后外周血淋巴细胞亚群及炎症因子表达改变及临床意义。方法:回顾分析2022年12月8日-2023年6月28日期间就诊于广西壮族自治区南溪山医院的52例新冠感染患者临床资料,按核酸检测结果阳性和转阴两个节点将患者分为入院阳性组和治疗转阴组,采用血细胞仪检测外周血淋巴细胞绝对计数,流式细胞术检测淋巴细胞亚群CD3^(+)CD4^(+)T、CD3^(+)CD8^(+)T绝对值,全自动荧光免疫分析仪检测SAA、CRP和IL-6含量,分析以上指标与患者临床特征间的关系。结果:新冠感染患者入院阳性组与治疗转阴组比较,外周血淋巴细胞绝对计数、淋巴细胞亚群CD3^(+)CD4^(+)T、CD3^(+)CD8^(+)T均有不同程度降低(P均<0.01),外周血SAA、CRP和IL-6均有不同程度升高(P均<0.01)。结论:新冠感染患者外周血T淋巴细胞亚群分析和炎症指标的改变与病情转归相关,可通过监测以上指标,了解机体当时免疫力水平和临床症状严重程度,预测疗效,为临床后续治疗提供理论依据。
Objective:To explore the expression and clinical significance of peripheral blood lymphocyte subsets and inflammatory factors in patients who were infected with corona virus disease 2019(COVID-19)and the severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)nucleic acid converted to negative.Methods:A total of 52 patients who infected with COVID-19 in Nanxishan Hospital of Guangxi Zhuang Autonomous Region from December 8,2022 to June 28,2023 were selected as the research objects.According to the results of positive or negative to SARS-CoV-2 nucleic acid,patients were divided into hospitalized positive group and treatment negative group.Automatic hematology analyzer was used to detect the absolute peripheral lymphocyte count,flow cytometry was used to detect the absolute counts of T lymphocyte subsets(CD3^(+)CD4^(+)T lymphocytes,CD3^(+)CD8^(+)T lymphocytes)and serum amyloid A(SAA),Creaction protein(CRP),interleukin-6(IL-6)were detected by Vitek immune diagnostic assay system in each group.Further,we analyzed the relationship between the changes in above indicators and the clinical characteristics in patients.Results:Compared with the treatment negative group,the positive group peripheral blood lymphocyte subsets,CD3^(+)CD4^(+)T lymphocytes,CD3^(+)CD8^(+)T lymphocytes were significantly decreased(P<0.01);SAA,CRP and IL-6 were increased(P<0.05).Conclusion:The levels of peripheral blood T lymphocyte subsets,SAA,CRP and IL-6 in COVID-19 patients were closely related to the severity of symptoms,monitoring the above indicators might help us to evaluate the severity of the disease and predict the prognosis.
作者
万颖
孙天寿
郑又文
WAN Ying;SUN Tian-shou;ZHENG You-wen(Nanxishan Hospital of Guangxi Zhuang Autonomous Region(The Second peoples Hospital of Guangxi Zhuang Autonomous Region),Guangxi Guilin 541002)
出处
《医学检验与临床》
2024年第3期11-14,共4页
Medical Laboratory Science and Clinics
基金
血清淀粉样蛋白A在多发骨髓瘤早期诊断及病情评估和预后的应用价值,项目编号:Z20201465
血清淀粉样蛋白A1(SAA1)在前列腺癌中的表达及临床研究,项目编号:Z20201493。
关键词
新型冠状病毒
淋巴细胞亚群
炎症因子
Novel coronavirus
T lymphocyte subsets
Inflammatory factors
作者简介
通讯作者:郑又文,E-mail:19327872@qq.com。
