摘要
肥胖是一种普遍存在的疾病,与许多严重的并发症有关。为了探究香榧油对肥胖小鼠脂质代谢影响的潜在分子机制,以高脂饮食建立肥胖小鼠模型,评估香榧油的降脂、抗氧化和抗炎症功能。结果表明,与高脂饮食(high-fat diet,HFD)组小鼠相比,香榧油高剂量(Torreya seed oil high-dose,SCH)组小鼠总胆固醇、甘油三酯和低密度脂蛋白胆固醇的水平均显著降低,高密度脂蛋白胆固醇的水平显著升高(P<0.05)。香榧油能显著抑制体重增长,降低肝脏系数和肾周脂肪积累。此外,香榧油干预后能降低肥胖小鼠血清中肿瘤坏死因子-α和白介素6水平,提高超氧化物歧化酶和谷胱甘肽过氧化酶酶活力,改善氧化应激和炎症反应。实时荧光定量PCR(real-time quantitative PCR,RT-PCR)结果显示,与HFD组相比,香榧油低剂量(Torreya seed oil low-dose,SCL)组小鼠固醇调节元件结合蛋白-1c(sterol regulatory element-binding protein-1c,SPEBP-1c)表达水平显著降低(P<0.05),而腺苷酸活化蛋白激酶α(adenosine monophosphate(AMP)-activated protein kinase alpha,AMPKα)和脂肪酸合酶(fatty acid synthase,FAS)的表达水平略有降低但不显著;SCH组小鼠AMPKα、SPEBP-1c和FAS表达均显著降低(P<0.05)。香榧油改善肥胖小鼠脂质代谢紊乱的作用机制可能是通过调节AMPKα/SREBP-1c信号通路,提高抗氧化水平和降低炎症反应实现。
Obesity is a widespread disease associated with many serious complications.This study aimed to investigate the potential molecular mechanisms of Torreya seed oil on lipid metabolism in obese mice,an obese mice model established with a high-fat diet to evaluate the hypolipidemic,antioxidant,and anti-inflammatory functions.The levels of total cholesterol,triglycerides,and low-density lipoprotein cholesterol were significantly decreased,but the level of high-density lipoprotein cholesterol was significantly increased in the Torreya seed oil high-dose(SCH)group mice compared with the high-fat diet(HFD)group mice(P<0.05).Meanwhile,Torreya seed oil could significantly reduce weight gain and decrease liver and perirenal fat accumulation.Torreya seed oil also reduced the expression levels of tumor necrosis factor-αand interleukin-6,increased the activities of superoxide dismutase and glutathione peroxidase,and improved oxidative stress and inflammatory response.Real-time quantitative PCR(RT-PCR)showed that the expression level of SPEBP-1c in the Torreya seed oil low-dose group was significantly reduced(P<0.05),while the expression levels of adenosine monophosphate(AMP)-activated protein kinase alpha(AMPKα)and fatty acid synthase(FAS)were only slightly reduced.The expression levels of AMPKα,sterol regulatory element-binding protein-1c(SPEBP-1c),and FAS in the SCH group were significantly decreased compared with those of the untreated obese mice(P<0.05).These results suggested that Torreya seed oil improved lipid metabolism disorders by regulating the AMPKα/SREBP-1c signalling pathway expression,increasing antioxidant levels,and reducing inflammatory responses in obese mice.
作者
蒋起宏
姚诗炜
卢红伶
蒋陈凯
胡文君
冯永才
陈振滨
沈国新
相兴伟
陈琳
JIANG Qihong;YAO Shiwei;LU Hongling;JIANG Chenkai;HU Wenjun;FENG Yongcai;CHEN Pingtan;SHEN Guoxin;XIANG Xingwei;CHEN Lin(Zhejiang Academy of Agricultural Sciences,Institute of Sericultural and Tea,Hangzhou 310021,China;Zhejiang University of Technology,College of Food Science and Technology,Huzhou 313200,China;Zhuji LYUkang Biotechnology Co.Ltd.,Shaoxing 311800,China)
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2024年第8期176-181,共6页
Food and Fermentation Industries
基金
浙江省中西医结合肿瘤防治技术研究重点实验室开放课题基金(202208CL)
浙江省农业科学院成果示范推广项目。
关键词
香榧油
肥胖
脂质代谢
高脂血症
Torreya seed oil
obesity
lipid metabolism
hyperlipoidemia
作者简介
第一作者:蒋起宏,硕士研究生;通信作者:陈琳,副研究员,E-mail:chenlinsdau@163.com。
