摘要
四指马鲅(Eleutheronema tetradactylum)为名贵海产鱼类,因其对应激反应强烈,易在运输过程中受损。热休克蛋白70(heat shock protein 70,HSP70)作为一种热休克蛋白(heat shock protein,HSP),是HSP家族中最重要的一员,对细胞蛋白质折叠过程及细胞对压力的适应起着重要作用。比较四指马鲅仔鱼和幼鱼在运输胁迫下的抗氧化能力和应激反应,进行幼鱼鳃组织学观察,探究热休克蛋白HSP70基因的组织表达模式以及在仔鱼和幼鱼中的表达差异,研究验证了运输胁迫下仔鱼和幼鱼阶段四指马鲅的抗氧化能力和HSP70表达呈现显著差异,并探讨了其作为评估抗应激反应及养殖管理效果的生物指标的潜力。
Eleutheronema tetradactylum is a valuable marine fish,which is susceptible to damage during transportation due to its strong response to stress.As a heat shock protein(HSP),HSP7O,the most important member of the HSP family,plays a crucial role in cellular protein folding and cellular adaptation to stress.By comparing the antioxidant capacity and stress response of the larval and juvenile fish under transportation stress,histological observations of juvenile gill tissues were conducted to explore the tissue expression patterns of the heat shock protein HSP70 gene and the expression differences between larvae and juveniles.Significant differences in antioxidant capacity and HSP70 expression between larval and juvenile Eleutheronema tetradactylum under transportation stress were confirmed,thus highlighting the potential of HSP70 expression as biological indicators for assessing stress response and aquaculture management effectiveness.
作者
吴子宜
赖文杰
邹振江
宋亚康
黄舜梅
汤胜亮
卢丹琪
张勇
WU Ziyi;LAI Wenjie;ZOU Zhenjiang;SONG Yakang;HUANG Shunmei;TANG Shengliang;LU Danqi;HANG Yong(School of Life Sciences,Sun Yat-sen University,Guangzhou 510275,China;Guangdong Provincial Key Laboratory for Aquatic Economic Animals,Sun Yat-sen University,Guangzhou 510275,China;Southern Marine Science and Engineering Guangdong Laboratory(Zhuhai),Zhuhai Guangdong 519000,China;Zhuhai Longsheng Improved Fingerling Breeding Co Ltd.,Zhuhai Guangdong 519090,China)
出处
《海南热带海洋学院学报》
2024年第2期1-10,37,共11页
Journal of Hainan Tropical Ocean University
基金
广东省科技计划项目(2023B1212060023)
南方海洋科学与工程广东省实验室(珠海)科研项目(SML2023SP201)。
关键词
四指马鲅
运输胁迫
抗氧化能力
HSP70
表达分析
Eleutheronema tetradactylum
transportation stress
antioxidant capacity
HSP70
expression analysis
作者简介
第一作者:吴子宜,女,广东梅州人,在读硕士研究生,研究方向为鱼类生物技术与工程;通信作者:张勇,男,江西抚州人,教授,博士,博士生导师,研究方向为水生经济动物繁殖与鱼类育种。
