摘要
金针菇多糖(Flammulina velutipes polysaccharide,FVP)具有良好的抗炎、抗氧化作用。为进一步探讨FVP缓解血管损伤的效果和作用机制,构建苯并[a]芘(benzo[a]pyrene,Ba P)小鼠染毒模型,通过免疫组化检测血管炎症因子表达量;使用流式细胞术分析外周血和脾脏中促炎细胞比例及骨髓造血细胞亚群构成。结果显示,FVP处理缓解Ba P诱导的白介素-6、单核细胞趋化蛋白-1和细胞间黏附分子-1等血管促炎因子的表达。此外,FVP处理显著抑制Ba P诱发的小鼠外周血中单核细胞、巨噬细胞和中性粒细胞以及脾脏中巨噬细胞和中性粒细胞比例的增加。进一步分析骨髓造血细胞亚群构成发现,Ba P处理后增加了小鼠骨髓中造血祖细胞和粒细胞巨噬细胞祖细胞的比例,减少了造血干细胞和普通髓系祖细胞的比例,而FVP处理后逆转了这一现象,使骨髓造血细胞亚群恢复至健康对照水平。同时细胞水平上的研究表明,FVP可抑制Ba P诱导Raw264.7细胞产生的氧化应激。综上,FVP可能是通过维持骨髓造血系统的稳态、调节促炎细胞向循环系统的输出和下调外周促炎细胞的比例缓解Ba P引发的血管炎症损伤。
Flammulina velutipes polysaccharides(FVP)have been reported to exhibit excellent anti-inflammatory and antioxidant effects.This study further investigated the efficacy and mechanism of action of FVP in alleviating vascular injury induced by benzo[a]pyrene(BaP)in mice.The expression of vascular inflammatory cytokines was assessed through immunohistochemistry analysis.Flow cytometry was used to analyze the proportion of pro-inflammatory cells in the peripheral blood and spleen as well as the composition of hematopoietic stem cell(HSC)subsets in bone marrow.The results demonstrated that FVP treatment mitigated the expression of vascular pro-inflammatory cytokines such as interleukin-6,monocyte chemoattractant protein-1 and intercellular cell adhesion molecule-1 in mice induced by BaP.Moreover,FVP treatment significantly inhibited the increases in the proportions of monocytes,macrophages and neutrophils in the peripheral blood and those of macrophages and neutrophils in the spleen of BaP-induced mice.Further analysis of bone marrow HSC subsets revealed that BaP exposure elevated the proportions of hematopoietic progenitor cells and granulocyte-macrophage progenitor cells in mouse bone marrow,while reducing the proportions of hematopoietic stem cells and common myeloid progenitor cells,which were reversed by FVP treatment,thus restoring bone marrow HSC subsets to the level of the healthy control group.At the cellular level,FVP suppressed BaP-induced oxidative stress in Raw264.7 cells.Taken together,FVP alleviate BaP-induced vascular inflammatory injury by maintaining the homeostasis of the bone marrow hematopoietic system,thereby regulating the output of pro-inflammatory cells into the circulatory system,and down-regulating the proportion of peripheral pro-inflammatory cells.
作者
牛泽淼
李鑫
陈宁
梁振华
赵娟
吕品
张岩
付程浩
NIU Zemiao;LI Xin;CHEN Ning;LIANG Zhenhua;ZHAO Juan;LÜPin;ZHANG Yan;FU Chenghao(School of Basic Medicine,Hebei Medical University,Shijiazhuang 050017,China;College of Pharmacy,Hebei Medical University,Shijiazhuang 050017,China;Experimental Center for Teaching,Hebei Medical University,Shijiazhuang 050017,China;Hebei Food Safety Key Laboratory,Hebei Food Inspection Institute,Shijiazhuang 050227,China)
出处
《食品科学》
EI
CAS
CSCD
北大核心
2024年第8期105-113,共9页
Food Science
基金
国家自然科学基金面上项目(32272328)
河北医科大学研究生创新资助项目(XCXZZS202310)
河北省教育厅青年科学基金项目(QN2023229)
河北省人社厅择优资助博士后科研项目(B2022003031)。
关键词
金针菇多糖
苯并[A]芘
血管炎症
造血作用
髓系细胞生成
Flammulina velutipes polysaccharides
benzo[a]pyrene
vascular inflammation
hemopoiesis
myeloid cell generation
作者简介
第一作者:牛泽淼(1999-)(ORCID:0009-0008-4062-7963),女,硕士,研究方向为血管重塑性疾病的发病机制。E-mail:546857723@qq.com;通信作者:张岩(1979-)(ORCID:0000-0002-2947-1629),男,正高级工程师,博士,研究方向为食品安全。E-mail:snowwinglv@126.com;通信作者:付程浩(1991-)(ORCID:0000-0002-0526-3470),男,讲师,博士,研究方向为炎症和血管重塑性疾病。E-mail:fch4059@163.com。
