摘要
Low temperature causes rice yield losses of up to 30%–40%,therefore increasing its cold tolerance is a breeding target.Few genes in rice are reported to confer cold tolerance at both the vegetative and reproductive stages.This study revealed a rice-specific 24-nt miRNA,miR1868,whose accumulation was suppressed by cold stress.Knockdown of MIR1868 increased seedling survival,pollen fertility,seed setting,and grain yield under cold stress,whereas its overexpression conferred the opposite phenotype.Knockdown of MIR1868 increased reactive oxygen species(ROS)scavenging and soluble sugar content under cold stress by increasing the expression of peroxidase genes and sugar metabolism genes,and its overexpression produced the opposite effect.Thus,MIR1868 negatively regulated rice cold tolerance via ROS scavenging and sugar accumulation.
基金
supported by grants from the National Natural Science Foundation of China(U20A2025,32101672,31971826)
the National Key Research and Development Plan of China(2021YFF1001100)
Natural Science Foundation of Heilongjiang province(YQ2023C035)
Double First-class Innovation Achievement Program of Heilongjiang Province(LJGXCG2023-072)
the Graduate Student Scientific Research Innovation Projects of Heilongjiang Bayi Agricultural University(YJSCX2022-Z01)。
作者简介
Corresponding authors:Mingzhe Sun,E-mail addresses:sunmingzhe@byau.edu.cn;Corresponding authors:Xiaoli Sun,sunxiaoli2016@byau.edu.cn。
