摘要
建立基质固相分散萃取-超高效液相色谱-串联质谱法检测眼影和眉笔中苯氧乙醇、脱氢乙酸、山梨酸、甲基氯噻唑琳酮和甲基氯异噻唑啉酮5种防腐剂的方法。样品与分散剂佛罗里硅土共研磨后,装入萃取小柱,用甲醇-丙酮洗脱萃取,对样品中防腐剂进行提取。采用超高效液相色谱-串联质谱的多反应监测模式(MRM)对5种防腐剂进行定性定量检测。这5种防腐剂在1~200μg/L均呈良好的线性关系,相关系数(R)均大于0.995,该方法检出限为0.003~0.008μg/g,定量限为0.009~0.024μg/g。该方法具有分离快速、操作简单、灵敏度高、重现性好、线性范围宽等优点,能解决眼影、眉笔等黏性易结块样品溶解不完全导致检测结果偏低的问题。
A matrix solid-phase dispersion extraction-ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)method was developed for the determination of five preservatives,namely,phenoxyethanol,dehydroacetic acid,sorbic acid,methylchlorothiazolinone,and methylchloroisothiazolinone,in eyeshadow and eyebrow pencil.The samples were co-milled with the dispersant Florian silica and loaded onto an extraction cartridge,and extracted with methanol-acetone elution to extract the preservatives from the samples.The 5 preservatives were qualitatively and quantitatively detected by ultra performance liquid chromatography-tandem mass spectrometry in multiple reaction monitoring(MRM)mode.The method showed good linearity in the range of 1-200μg/L with the correlation coefficients(R)greater than 0.995.The limits of detection of the method were 0.003-0.008μg/g,and the limits of quantification were 0.009-0.024μg/g.The method has the advantages of rapid separation,simple operation,high sensitivity,good reproducibility,and wide linear range,which can solve the problem of low detection caused by incomplete dissolution of viscous and easily agglomerated samples such as eyeshadow and eyebrow pencil.
作者
黄运龙
华腾辉
姚天波
梁龙
周宏斌
袁剑辉
郑琳
HUNAG Yunlong;HUA Tenghui;YAO Tianbo;LIANG Long;ZHOU Hongbin;YUAN Jianhui;ZHENG Lin(Ningbo Joysun Product Testing Service Company,Ningbo 315000,Zhejiang,China;Ningbo Eyecos Co.,Ltd.,Ningbo 315000,Zhejiang,China;Ningbo Institute of Inspection and Quarantine Science and Technology,Ningbo 315048,Zhejiang,China)
出处
《香料香精化妆品》
CAS
2024年第2期142-146,共5页
Flavour Fragrance Cosmetics
基金
宁波市公益计划项目(编号2023S121)
宁波科技服务项目(编号2020F023)。
关键词
基质固相分散萃取
防腐剂
超高效液相色谱-串联质谱法
眼影
眉笔
matrix solid-phase dispersive extraction
preservative
ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)
eyeshadow
eyebrow pencil
作者简介
黄运龙(1993-),男,硕士,工程师,主要从事化妆品检测研究;通信作者:郑琳(1976-),女,硕士,高级工程师,主要从事化妆品质量安全检测与研究。电话:0574-87022660,E-mail:zhenglinlxl@163.com。
