摘要
昆虫几丁质酶可以降解昆虫体壁和围食膜中的几丁质,在昆虫蜕皮等生命活动中发挥重要功能。作为参与昆虫几丁质降解系统的关键酶,Ⅱ家族几丁质酶(ChtⅡ)是少见的多结构域、性质复杂的几丁质酶。对昆虫中编码ChtⅡ的基因沉默会导致昆虫蜕皮失败及死亡率增加。对ChtⅡ的生化性质及晶体结构分析表明,其在几丁质降解系统中起到了“先锋及攻坚”的作用。因此,ChtⅡ有望成为新的杀虫剂靶标。本文综述了近年来关于ChtⅡ的生理功能、结构特征及其抑制剂的研究成果,可为基于昆虫几丁质酶的新杀虫剂创制提供参考。
Insect chitinases can degrade chitin in the insect body wall and peritrophic membrane,thus playing a crucial role in insect molting and other life activities.Among the key enzymes involved in the chitin degradation system of insects,group II chitinase(ChtII)is a specific enzyme with multiple domains and complex functions.Silencing of the gene encoding ChtII in insects can lead to molting failure and increased mortality.The analysis of the biochemical properties and crystal structure of ChtII indicates its"pioneering and tackled"role in the chitin degradation system.Thus,ChtII is expected to be a new target for insecticides.This paper reviews recent progress on the physiological function,structural characteristics,and inhibitors of ChtII,aiming to provide insights for the creation of green pesticides based on insect chitinases.
作者
谢慧杰
丁艺
胡雪
杨青
刘田
XIE Huijie;DING Yi;HU Xue;YANG Qing;LIU Tian(School of Bioengineering,Dalian University of Technology,Dalian 116024,Liaoning Province,China;Genome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs,Agricultural Genomics Institute,Chinese Academy of Agricultural Sciences,Shenzhen 518120,Guangdong Province,China;State Key Laboratory for Biology of Plant Diseases and Insect Pests,Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing 100193,China)
出处
《农药学学报》
CAS
CSCD
北大核心
2024年第2期224-233,共10页
Chinese Journal of Pesticide Science
基金
国家重点研发计划(2022YFD1700200)
国家自然科学基金(32170502)
辽宁省自然科学基金(2022-KF-15-02).
作者简介
第一作者:谢慧杰,xiehuijie@mail.dlut.edu.cn;通讯作者:刘田,tianliu@dlut.edu.cn.
