摘要
目的测定不同黄柏炮制品中主要成分黄柏碱和小檗碱之间的含量差异,以探讨不同炮制方法对该药材质量的影响。方法采用高效液相色谱法(HPLC)对不同黄柏炮制品中的黄柏碱和小檗碱进行含量测定,以Diamonsil C_(18)(250 mm×4.6 mm,5µm)为色谱柱,乙腈-0.1%甲酸为流动相,梯度洗脱,流速为1.0 ml·min^(-1),柱温25℃,检测波长为284 nm。结果黄柏碱和小檗碱线性范围分别为0.354~0.760、3.865~7.530 g·L^(-1),线性关系良好,均R2>0.9990。黄柏不同炮制品中黄柏碱和小檗碱含量差异较大,酒黄柏的黄柏碱与小檗碱含量最高,分别为1.360 mg·g^(-1)、10.200 mg·g^(-1),黄柏碱含量由低到高顺序为:黄柏炭<黄柏生品<盐黄柏<酒黄柏,小檗碱含量由低到高顺序为黄柏炭<盐黄柏<黄柏生品<酒黄柏。结论不同炮制方法会对黄柏的主要有效成分黄柏碱和小檗碱产生影响,且可能影响药效。本研究方法简便、科学合理,可为深入研究黄柏提供参考。
Objective To determine the content difference between the main componentsof Phellodendrine and Berberine in different processing methods,and to discuss the effect ofprocessing on the quality of Cortex Phellodendri.Methods The contents of Phellodendrine andBerberine in different processing methods were determined by high performance liquidchromatography(HPLC).Using Diamonsil C_(18)(250.0 mm×4.6 mm,5μm)as column,acetonitrile-0.1%formic acid as gradient elution,the flow rate was 1.0 ml·min^(-1),the column temperature was 25℃,and thedetection wavelength was 284 nm.Results Phellodendrine and Berberine were well related linearlywithin the mass concentration of 0.354-0.760,3.865-7.530 g·L^(-1)(R2>0.9990).The contents ofPhellodendrine and Berberine in different processed products of Cortex Phellodendri were different.The contents of Phellodendrine and Berberine in wine Phellodendri were the highest,which were1.360 mg·g^(-1)and 10.200 mg·g^(-1).The order from low to high of Phellodendrine content was as follows:Phellodendri charcoal<raw Phellodendri<salt Phellodendri<wine Phellodendri;the order fromlow to high of Berberine content was as follows:Phellodendri charcoal<salt Phellodendri<rawPhellodendri<wine Phellodendri.Conclusions Different processing methods affect the mainactive components of Phellodendrine and Berberine,and then its pharmacodynamics.The method issimple,scientific,and reasonable,which could be used for further study of Cortex Phellodendri.
作者
杨永乐
胡振宇
阿丽牙·阿布来提
叶喜德
Yang Yonge;Hu Zhenyu;ALIYA·Abuaiti;Ye Xide(Quality Control Department,Wannian County Hospital of Traditional Chinese Medicine,Shangrao 335500,China;College of Pharmacy,Jiangxi University of Chinese Medicine,Nanchang 330004,China)
出处
《国际医药卫生导报》
2024年第8期1372-1377,共6页
International Medicine and Health Guidance News
基金
江西省科技专项重点研发计划(20232BBG70013)。
关键词
黄柏
炮制
黄柏碱
小檗碱
高效液相色谱法
含量测定
Cortex Phellodendri
Processing
Phellodendrine
Berberine
HPLC
Content determination
作者简介
通信作者:叶喜德,Email:552376722@qq.com。
