摘要
目的探讨白细胞介素17(IL-17)信使RNA(mRNA)和前列腺素E2(PGE2)mRNA在口腔扁平苔藓(OLP)患者中的表达水平及临床意义。方法选取2020年3月至2021年3月宁夏医科大学总医院口腔医院收治的35例OLP患者作为试验组,另选取同期在宁夏医科大学总医院口腔医院进行阻生牙拔除的20例患者作为对照组,根据组织是否糜烂,将试验组患者分为糜烂型OLP组和非糜烂型OLP组。检测并比较糜烂型OLP组和非糜烂型OLP组患者口腔黏膜病损组织及对照组患者口腔黏膜组织的IL-17 mRNA和PGE2 mRNA表达水平,并通过视觉模拟评分法(VAS)评估OLP患者的疼痛程度,比较不同疼痛程度的OLP患者IL-17 mRNA和PGE2 mRNA表达水平。采用Pearson相关分析OLP患者IL-17 mRNA与PGE2 mRNA表达水平的相关性。采用Spearman相关分析OLP患者IL-17 mRNA和PGE2 mRNA表达水平与疼痛程度的相关性。结果非糜烂型OLP组有19例患者,糜烂型OLP组有16例患者。糜烂型OLP组IL-17 mRNA和PGE2 mRNA表达水平均高于非糜烂型OLP组和对照组,且非糜烂型OLP组均高于对照组,差异均有统计学意义(P<0.05)。试验组中无疼痛患者有5例,轻度疼痛患者有6例,中度疼痛患者有16例,重度疼痛患者有8例。无疼痛OLP患者IL-17 mRNA和PGE2 mRNA表达水平均低于轻度、中度、重度疼痛OLP患者,差异均有统计学意义(P<0.05)。Pearson相关分析结果显示,OLP患者IL-17 mRNA表达水平与PGE2 mRNA表达水平呈正相关(r=0.505,P=0.002)。Spearman相关分析结果显示,OLP患者IL-17 mRNA、PGE2 mRNA表达水平与疼痛程度均呈正相关(r=0.710、0.570,P<0.05)。结论IL-17 mRNA和PGE2 mRNA参与了OLP的发生、发展过程,并且其表达水平与OLP患者疼痛程度呈正相关。
Objective To investigate the expression and clinical significance of interleukin-17(IL-17)mRNA and prostaglandin E2(PGE2)mRNA in patients with oral lichen planus(OLP).Methods A total of 35 OLP patients admitted to the Stomatological Hospital,General Hospital of Ningxia Medical University from March 2020 to March 2021 were selected as the experimental group,and 20 patients who underwent impacted tooth extraction in the Stomatological Hospital,General Hospital of Ningxia Medical University during the same period were selected as the control group.The patients in the experimental group were divided into erosive OLP group and non-erosive OLP group according to whether the tissue was erosive or not.The expression levels of IL-17 mRNA and PGE2 mRNA in the oral mucosal lesion tissues of the erosive OLP group and the non-erosive OLP group and the control group were detected and compared,and the pain degree of OLP patients was evaluated by visual analogue scale(VAS).The expression levels of IL-17 mRNA and PGE2 mRNA in OLP patients with different degrees of pain were compared.Pearson correlation analysis was used to analyze the correlation between the expression levels of IL-17 mRNA and PGE2 mRNA in patients with OLP.Spearman correlation analysis was used to analyze the correlation between the expression levels of IL-17 mRNA and PGE2 mRNA and the degree of pain in patients with OLP.Results There were 19 patients in non-erosive OLP group and 16 patients in erosive OLP group.The expression levels of IL-17 mRNA and PGE2 mRNA in erosive OLP group were higher than those in non-erosive OLP group and control group,and those in non-erosive OLP group were higher than control group,and the differences were statistically significant(P<0.05).In the experimental group,there were 5 patients with no pain,6 patients with mild pain,16 patients with moderate pain,and 8 patients with severe pain.The expression levels of IL-17 mRNA and PGE2 mRNA in OLP patients without pain were lower than those in OLP patients with mild,moderate and severe pain,and the differences were statistically significant(P<0.05).Pearson correlation analysis showed that the expression level of IL-17 mRNA correlated positively with the expression level of PGE2 mRNA in OLP patients(r=0.505,P=0.002).Spearman correlation analysis showed that the expression levels of IL-17 mRNA and PGE2 mRNA in OLP patients correlated positively with the degree of pain(r=0.710,0.570,P<0.05).Conclusion The expression of IL-17 mRNA and PGE2 mRNA is involved in the occurrence and development of OLP,and its expression level correlates positively with the degree of pain in patients with OLP.
作者
李阳
王珑
LI Yang;WANG Long(School of Stomatology,Ningxia Medical University,Yinchuan,Ningxia 750001,China)
出处
《检验医学与临床》
CAS
2024年第8期1103-1106,共4页
Laboratory Medicine and Clinic
基金
宁夏回族自治区高等学校科学研究项目(NGY2020039)。
作者简介
李阳,男,主治医师,主要从事口腔黏膜病的诊断和治疗方面的研究;通信作者:王珑,E-mail:571549896@qq.com。
