摘要
目的明确佛芍颗粒对功能性腹痛模型小鼠的治疗作用,为临床应用提供基础研究数据支持。方法实验小鼠按体质量随机分为正常对照组、模型对照组、吗丁啉对照组(12 mg·kg^(-1))、四逆散对照组(3 g·kg^(-1))、佛芍颗粒高剂量组(48 g生药·kg^(-1))、佛芍颗粒中剂量组(24 g生药·kg^(-1))、佛芍颗粒低剂量组(12 g生药·kg^(-1))7组,每组10只,雌雄各半。采用寒冷刺激结合腹腔注射福尔马林制备寒凝气滞功能性腹痛病证结合模型,通过检测小鼠疼痛潜伏期、疼痛时长、疼痛发生率及结肠组织病理变化等指标综合评价佛芍颗粒对功能性腹痛小鼠的治疗作用。结果佛芍颗粒可以明显延长疼痛潜伏期(P<0.05,P<0.01)、缩短疼痛持续时间、降低疼痛发生率,减轻小鼠结肠黏膜增生及炎症等。结论佛芍颗粒可以改善寒冷刺激结合福尔马林诱导的寒凝气滞功能性腹痛病证结合模型小鼠的腹痛,为佛芍颗粒的临床应用提供参考。
Objective To clarify the therapeutic effect of Foshao granules in mice with spastic abdominal pain,and provide basic research data support for clinical application.Methods Experimental mice were randomly divided into seven groups according to their body weight:the normal control group,model control group,Domperidone control group(12 mg·kg^(-1)),Sini Powdered control group(3 g·kg^(-1)),Foshao granules high dosage group(48 g crude drug·kg^(-1)),Foshao granules middle dosage group(24 g crude drug·kg^(-1)),Foshao granules low dosage group(12 g crude drug·kg^(-1)),with 10 mice in each group.A cold stagnation functional abdominal painmedical syndrome combination model made by cold stimulation combined with an intraperitoneal injection of formalin.The therapeutic effect of mice with spastic abdominal pain induced by neostigmine was evaluated with the pain latency,the pain incidence,duration of pain,the pathological changes in the colon of the mice.Results The Foshao granules can significantly reduce the incidence of pain,prolong the pain incubation(P<0.05,P<0.01),shorten the pain length,and reduce the mucosal hyperplasia,and the enlargement and increase of goblet cells in the interstitial inflammation machine in the colon.Conclusion The Foshao granules significantly improved cold stimulation combined with formalin-induced functional abdominal pain in mice,providing a reference for the clinical application of Foshao granules.
作者
李舒冉
郭姗姗
包蕾
孙静
赵荣华
鲍岩岩
耿子涵
张宇
曹姗
吕耀中
王振中
崔晓兰
王红梅
LI Shuran;GUO Shanshan;BAO Lei;SUN Jing;ZHAO Ronghua;BAO Yanyan;GENG Zihan;ZHANG Yu;CAO Shan;LYU Yaozhong;WANG Zhenzhong;CUI Xiaolan;WANG Hongmei(Biosecurity Laboratory,Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China;National Key Laboratory of Process Control and Intelligent Manufacturing Technology of Traditional Chinese Medicine Pharmaceuticals,Lianyungang Jiangsu 222000,China;Jiangsu Kangyuan Pharmaceutical Co.,Ltd,Lianyungang Jiangsu 222000,China)
出处
《中国药物警戒》
2024年第3期269-272,共4页
Chinese Journal of Pharmacovigilance
基金
国家自然科学基金资助项目(82141206)
中央级公益性科研院所基本科研业务费专项资金资助(ZZ15-YQ-035,ZXKT21031)
中国中医科学院科技创新项目(CI2021B015)。
关键词
功能性腹痛
寒凝气滞证
佛芍颗粒
治疗作用
小鼠
neostigmine
spastic abdominal pain
Foshao granules
therapeutical effect
mice
作者简介
李舒冉,女,博士,助理研究员,中药防治感染性疾病机制研究;通信作者:崔晓兰,女,博士,研究员·博导,中药防治感染性疾病机制研究。E-mail:xlcui@icmm.ac.cn;共同通信作者:王红梅。
