摘要
筛选出滁菊枯萎病病原菌的拮抗菌株,可为滁菊枯萎病的防治提供菌株资源。使用连作滁菊根际土壤作为供试土壤分离微生物,经过CAS培养基和平板对峙试验筛选出产铁载体拮抗菌株。共分离纯化出183株细菌分离株,通过CAS培养基筛选出77株具有产铁载体功能的菌株,与尖孢镰刀菌平板对峙试验复筛出5株拮抗菌。其中TZ-8抑菌率为59.68%,且具备固氮、解磷、解钾和分泌IAA的能力,鉴定表明该菌株为越南伯克霍尔德氏菌(Burkholderia vietnamiensis)。侵染试验表明该菌株能够有效抵御病原菌侵染植物组织,盆栽试验表明该菌株对滁菊枯萎病具备良好的生防效果。通过侵染试验和盆栽试验验证拮抗菌株对滁菊枯萎病具有一定的防治效果,可纳入滁菊枯萎病生防菌剂的菌株资源库。
To screen for antagonistic bacteria against the pathogen causing Chuzhou chrysanthemum wilt disease and provide resources to control the disease.Microorganisms were isolated from the continuous cropping soil of Chuzhou chrysanthemum,and antagonistic strains with the ability to produce siderophores were selected through CAS medium and plate confrontation experiments.The antagonistic effects were verified through infection experiments and pot experiments on Chuzhou chrysanthemum wilt disease.A total of 183 bacterial isolates were purified from the soil,among which 77 strains with the ability to produce siderophores were selected using CAS medium,and 5 antagonistic strains were identified through confrontation culture with Fusarium oxysporum on plates.Among them,strain TZ-8 showed the highest inhibition rate of 59.68%,with the ability to fix nitrogen,solubilize phosphate and potassium,and secrete IAA.It was identified as Burkholderia vietnamiensis.The results of infection experiments showed that the strain could effectively resist pathogen infection in plant tissues,and the results of pot experiments demonstrated that the strain had a good biocontrol effect against Chuzhou chrysanthemum wilt disease.The strain TZ-8 can effectively control Chuzhou chrysanthemum wilt disease and can be included in the bacterial resource library for the biocontrol of the disease.
作者
吴瑞
陈雨婷
曹启荣
朱琳
周成
WU Rui;CHEN Yuting;CAO Qirong;ZHU Lin;ZHOU Cheng(College of Resource and Environment,Anhui Science and Technology University,Chuzhou 233100,Anhui,China;College of Life and Health Sciences,Anhui Science and Technology University,Chuzhou 233100,Anhui,China;College of Resources and Environmental Sciences,Nanjing Agricultural University,Nanjing 210095,Jiangsu,China)
出处
《江汉大学学报（自然科学版）》
2024年第2期13-19,共7页
Journal of Jianghan University：Natural Science Edition
基金
安徽省自然科学基金面上项目(2108085MC107)
安徽省重点研究与开发计划项目(202004a06020003)
安徽省教育厅高校科学研究重点项目(KJ2021A0893)
安徽省教育厅高校优秀青年人才支持计划一般项目(gxyq2022053)
大学生创新创业训练计划项目(S202110879052,202110879023)。
关键词
滁菊枯萎病
尖孢镰刀菌
根际促生菌
拮抗菌
生物防治
连作障碍
Chuzhou chrysanthemum wilt disease
Fusarium oxysporum
plant growthpromoting rhizobacteria(PGPR)
antagonistic bacteria
biological control
continuous cropping obstacle
作者简介
吴瑞(1996—),男,硕士生,研究方向:微生物-植物互作。;通信作者:周成(1985—),男,教授,博士,研究方向:微生物-植物互作。E-mail:zhoucheng@njau.edu.cn。
