摘要
明确枸杞籽油对中波紫外线(ultraviolet B,UVB)诱导人永生化角质形成细胞(human immortalized keratinocytes,HaCaT)光损伤的防护作用,进一步提高枸杞籽油的利用率。采用GB 5009.168—2016、GB 5009.83—2016、DB64/T 1514—2017、《保健食品中功效成分检测方法》和GB 5009.82—2016(第一法)提供的方法分别测定枸杞籽油主要活性成分脂肪酸、β-胡萝卜素、玉米黄质、叶黄素、谷甾醇以及维生素E的含量,利用HaCaT细胞构建UVB光损伤模型,检测枸杞籽油对UVB诱导的光损伤HaCaT细胞存活率、凋亡率、活性氧(reactive oxygen species,ROS)、凋亡相关细胞因子和炎症因子的影响。结果表明,与模型组相比,枸杞籽油可明显提高UVB光损伤HaCaT细胞的存活率(P<0.05),促进抗凋亡因子B淋巴细胞瘤/白血病-2 mRNA的表达(P<0.01),抑制细胞内ROS的生成(P<0.05),降低细胞凋亡率(P<0.01),抑制细胞凋亡相关因子Bcl-2相关X蛋白、半胱氨酸天冬氨酸蛋白酶-3、半胱氨酸天冬氨酸蛋白酶-9和p53 mRNA的表达(P<0.01),减少炎症因子肿瘤坏死因子-α、白细胞介素-1β和白细胞介素-6的蛋白含量及mRNA表达(P<0.05)。结果表明枸杞籽油可通过调控细胞凋亡及减轻炎症反应来缓解UVB所致HaCaT细胞光损伤。
This study aimed to investigate the protective effect of Lycium barbarum seed oil on ultraviolet B(UVB)-induced photodamaged human immortalized keratinocytes(HaCaT),and further improve the utilization of Lycium barbarum seed oil.The contents of fatty acid,carotenoid,sitosterol,and vitamin E were determined by the National industry standards,including GB 5009.168—2016,GB 5009.83—2016,DB 64/T 1514—2017,functional ingredients detection standards system of health food,and the first method of GB 5009.82—2016,respectively.The photodamage model of HaCaT cells was established by UVB irradiation.The cell viability,reactive oxygen species(ROS)content,apoptosis rate,mRNA expression of apoptosis-related cytokines and inflammatory factors,and content of inflammatory factors of photodamaged HaCaT cells were detected by CCK-8 method,2′,7′-dichlorofluorescein diacetate(DCFH-DA),flow cytometry,real-time fluorescence PCR(RT-PCR),and enzyme linked immunosorbent assay(ELISA),respectively.Results showed that,compared with the model group,Lycium barbarum seed oil could significantly improve the cell viability of photodamaged HaCaT cells(P<0.05),and promote the expression of the mRNA expression of Bcl-2(P<0.01).Furthermore,Lycium barbarum seed oil could significantly inhibit ROS production(P<0.05),decrease the apoptosis rate(P<0.01),down regulating the mRNA expressions of Bax,Caspase-3,Caspase-9,p53(P<0.01),and reduce the production of TNF-α,IL-1β,and IL-6 at both the expression and transcriptional levels(P<0.05)in the photodamaged HaCaT cells.In summary,Lycium barbarum seed oil could alleviate UVB-induced HaCaT cells photodamage by regulating apoptosis and alleviating the inflammatory response.
作者
王学红
尹星星
陆杰
张靖鹏
杨永晶
WANG Xuehong;YIN Xingxing;LU Jie;ZHANG Jingpeng;YANG Yongjing(School of Ecological and Environmental Engineering,Qinghai University,Xining 810016,China;Qinghai General Health Bio-science Co.Ltd.,Xining 810003,China)
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2024年第6期47-55,共9页
Food and Fermentation Industries
基金
“西部之光”人才培养计划项目
国家自然科学基金项目(81860619)
青海省科技厅基础研究项目(2019-ZJ-953Q)
青海大学生态环境工程学院研究生三江源一流学科科技创新项目(2022-stxy-Y12)。
关键词
枸杞籽油
人永生化角质形成细胞
光损伤
凋亡
炎症
Lycium barbarum seed oil
human immortalized keratinocytes
photodamage
apoptosis
inflammation
作者简介
第一作者:王学红,硕士研究生;通信作者:杨永晶,副教授,E-mail:yongjing223@163.com。
