摘要
目的采用网络药理学技术探究金匮肾气丸治疗阿尔茨海默病(AD)的潜在作用机制。方法利用TCMSP数据库,以口服生物利用度OB≥30%和类药性指数DL≥0.18筛选金匮肾气丸治疗AD的相关活性化学成分,组方化学成分靶点,通过Genecards数据库获取与AD疾病相关的靶标,使用韦恩分析筛选化学成分靶点与疾病靶点二者治疗AD的潜在作用靶点;采用Cytoscape构建构建“活性成分-作用靶点”作用网络图,采用R软件中的“ClusterProfiler”软件包进行GO和KEGG通路富集分析。结果共得到金匮肾气丸131个活性成分,216个化学成分靶点,如谷甾醇、玉兰脂素B、海风藤酮、汉辛酚、亚麻酸乙酯、泽泻醇B、黄连碱等;Genecards数据库获取到8718个AD相关蛋白基因;韦恩分析得到金匮肾气丸与AD的176个共同靶点,包括HSP90AA1、MAPK1、AKT1、JUN、RELA、FOS、ESR1、MAPK14、MYC等。GO分析显示共同靶点的生物功能主要涉及对氧化应激的反应、细胞对化学应激的反应、酰胺结合等过程;KEGG富集主要涉及体剪切应力与动脉粥样硬化、糖尿病并发症中的AGE-RAGE信号通路等。结论金匮肾气丸的主要活性成分为谷甾醇、玉兰脂素B、海风藤酮、汉辛酚、亚麻酸乙酯、泽泻醇B、黄连碱等,这些成分可通过HSP90AA1、MAPK1、AKT1、JUN、RELA、FOS、ESR1、MAPK14等关键靶点作用于体剪切应力与动脉粥样硬化、糖尿病并发症中的AGE-RAGE信号通路发挥治疗AD的作用。
Objective To explore the potential mechanism of Jingui Shenqi pills in the treatment of Alzheimer's disease(AD)by network pharmacology.Methods The TCMSP database was used to screen the active chemical components of Jinkui Shenqi pills in the treatment of AD with oral bioavailability OB≥30%and drug-like index DL≥0.18,and the chemical component targets were composed.The targets related to AD disease were obtained by Genecards database,and the potential targets of chemical component targets and disease targets in the treatment of AD were screened by Wayne analysis.Cytoscape was used to construct the"active ingredient-target"action network diagram,and the"ClusterProfiler"software package in R software was used for GO and KEGG pathway enrichment analysis.Results A total of 131 active ingredients and 216 chemical targets of Jinkui Shenqi pills were obtained,such as sitosterol,magnolin B,kadsura ketone,octylphenol,ethyl linolenic acid,alisol B,coptisine,etc.8718 AD-related protein genes were obtained from Genecards database.The 176 common targets of Jingui Shenqi pills and AD were obtained by Wayne analysis,including HSP90AA1,MAPK1,AKT1,JUN,RELA,FOS,ESR1,MAPK14,MYC,etc.GO analysis showed that the biological functions of the common targets were mainly involved in the response to oxidative stress,cell response to chemical stress,amide binding and other processes.KEGG enrichment mainly involved AGE-RAGE signaling pathway in body shear stress,atherosclerosis and diabetic complications.Conclusion The main active components of Jinkui Shenqi pill are sitosterol,magnolin B,kadsura ketone,wogonol,ethyl linolenic acid,alisol B,coptisine,etc.These components can play a role in the treatment of AD by HSP90AA1,MAPK1,AKT1,JUN,RELA,FOS,ESR1,MAPK14 and other key targets in the body shear stress and AGE-RAGE signaling pathway in atherosclerosis and diabetic complications.
作者
罗燕燕
季文莹
孙倩
马琴国
张柏桃
王婷婷
LUO Yan-yan;JI Wen-ying;SUN Qian;MA Qin-guo;ZHANG Bo-tao;WANG Ting-ting(Pharmacy Department of Gansu Provincial Hospital of Traditional Chinese Medicine,Lanzhou 730050,Gansu,China;Pharmacy Department of the Second Hospital of Lanzhou University,Lanzhou 730030,Gansu,China;Cardiac Function Examination Department of Gansu Provincial Hospital of Traditional Chinese Medicine,Lanzhou 730050,Gansu,China;Gansu Provincial Institute of Drug Control,Lanzhou 730070,Gansu,China)
出处
《医学信息》
2024年第6期22-27,共6页
Journal of Medical Information
基金
中医药人才培养重点学科项目(编号:甘财社〔2022〕48号)。
关键词
金匮肾气丸
阿尔茨海默病
网络药理学
Jinkui Shenqi pill
Alzheimer's disease
Network pharmacology
作者简介
罗燕燕(1992.1-),女,甘肃武威人,硕士,中药师,主要从事中药制剂工艺研究;通讯作者:王婷婷(1986.7-),女,甘肃庆阳人,硕士,主管中药师,主要从事中药品种鉴定与质量控制研究。
