摘要
目的建立采用抑制电导离子色谱法测定醋酸钙颗粒中钙离子的方法。方法采用Dionex IonPacTMCS12A色谱柱(250 mm×4 mm),Dionex IonPacTMCG12A保护柱(50 mm×4 mm),以0.02 mol/L甲烷磺酸溶液为洗脱液,体积流量1.0m L/min,柱温30℃,检测池温度35℃,电导检测器,抑制器为Dionex CDRS 6004 mm,电流值59 mA,进样体积为25μL。结果钙在0.002~6.000μg/mL线性关系良好(r=0.9997),平均回收率为104.6%,RSD值为1.8%。结论方法简单、专属性高、准确可靠,能够有效地控制醋酸钙颗粒中钙离子。
Objective To establish a method of ion chromatography-suppressed conductivity to determine calcium ion in Calcium Acetate Granules.Methods The assay was performed on Dionex IonPacTM CS12A column(250 mm×4 mm)with Dionex IonPacTM CG12A column(50 mm×4 mm)as guard column.Mobile phase was used as 0.02 mol/L methanesulfonic acid solution.Flow rate was 1.0 mL/min,column temperature was 30℃,and detecting pool temperature was 35℃.Conductivity detector was used with CDRS 6004 mm as suppressor,current value was 59 mA,and injection volume was 25μL.Results Calcium showed good linear relationships of 0.002—6.000μg/mL(r=0.9997),whose average recovery was 104.6%with RSD values of 1.8%.Conclusion The method is simple,highly specific,accurate and reliable,and can effectively control calcium ion in Calcium Acetate Granules.
作者
葛薇薇
吴珺
朱静
GE Weiwei;WU Jun;ZHU Jing(Taizhou Institute for Drug Control,Taizhou 225300,China)
出处
《现代药物与临床》
CAS
2024年第2期353-356,共4页
Drugs & Clinic
关键词
醋酸钙颗粒
钙
抑制电导离子色谱法
Calcium Acetate Granules
calcium
ion chromatography-suppressed conductivity
作者简介
葛薇薇,女,副主任药师,从事药物分析工作。E-mail:171484225@qq.com;通信作者:朱静,女,副主任药师,硕士,从事药物分析工作。E-mail:jingzhz@126.com。
