摘要
建立在乳粉、牛乳2种基质中超高效液相色谱-串联质谱法同时检测伏马毒素B_(1)(fumonisin B_(1),FB_(1))、FB_(2)和FB_(3)残留的检测方法。样品以体积分数50%乙腈溶液提取,稀释后经免疫亲和柱净化,甲醇-乙酸(98∶2,V/V)洗脱,氮吹后复溶,采用电喷雾离子源正离子多反应监测模式进行检测,同位素内标法定量。结果表明:本方法牛乳、乳粉基质中FB_(1)、FB_(2)和FB_(3)定量限均为10μg/kg,药物加标量10~400μg/kg时,加标回收率为92.8%~107.5%,相对标准偏差为2.0%~4.3%,基质效应为96.25%~122.84%。该方法具有检测周期短、适用于多种婴幼儿食品检测的特点。
An ultra-high performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)method for the simultaneous quantitative determination of fumonisin B_(1)(FB_(1)),fumonisin B_(2)(FB_(2))and fumonisin B_(3)(FB_(3))residues in milk powder and milk has been established.The samples were extracted with methanol-acetic acid(98:2,V/V),diluted,cleaned up by immunoaffinity chromatography(IAC),and blown to dryness under nitrogen.The residue was re-dissolved and detected using an electrospray ionization source(EIS)in the positive ion mode with multiple reaction monitoring(MRM)and quantitative analysis was performed by the isotope-labeled internal standard method.The limit of quantification was 10μg/kg for FB_(1),FB_(2) and FB_(3) in milk powder and milk.The recoveries at spiked concentration levels of 10–400μg/kg were 92.8%–107.5%,with relative standard deviations(RSD)of 2.0%–4.3%.The matrix effects were 96.25%–122.84%.This method was characterized by short analysis time and was suitable for the determination of fumonisin residues in different dairy products.
作者
莫楠
张立佳
高玉杰
白艳梅
汪洋
刘丽君
李翠枝
MO Nan;ZHANG Lijia;GAO Yujie;BAI Yanmei;WANG Yang;LIU Lijun;LI Cuizhi(Inner Mongolia Yili Industrial Group Co.Ltd.,Hohhot 010110,China)
出处
《乳业科学与技术》
2023年第6期34-39,共6页
JOURNAL OF DAIRY SCIENCE AND TECHNOLOGY
基金
内蒙古自治区重点研发和成果转化计划项目(2023YFHH0093)。
关键词
超高效液相色谱-串联质谱
伏马毒素类
真菌毒素
婴幼儿食品
乳粉
牛乳
ultra-high performance liquid chromatography-tandem mass spectrometry
fumonisins
mycotoxin
infant food
milk powder
milk
作者简介
第一作者:莫楠(1989-)(ORCID:0009-0003-5690-2847),男,工程师,学士,研究方向为乳制品检测分析。E-mail:jtmonan@yili.com;通信作者:李翠枝(1969-)(ORCID:0000-0002-1997-7917),女,教授级高级工程师,硕士,研究方向为食品安全管理和检验检测技术。E-mail:lczg@yili.com。
